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Detection of aberrant DNA methylation in colorectal carcinoma samples compared to normal human colon

ABSTRACT: To globally define methylation-’prone’ and -’protected’ CpG islands in colorectal carcinoma we analyzed the methylation status of 23,000 CpG islands of the human genome in ten coleorectal carcinoma samples as well as normal colon using our previously described methyl-CpG immunoprecipitation (MCIp) technique (Gebhard et al. 2006; Schilling and Rehli 2007). This method enriches for highly CpG methylated DNA that can be directly applied to fluorescent labeling and oligonucleotide microarray hybridization without an additional amplification step. Keywords: MCIp-on-Chip; comparative genomic hybridization

ORGANISM(S): Homo sapiens

PROVIDER: GSE17512 | GEO | 2010/03/31

SECONDARY ACCESSION(S): PRJNA118783

REPOSITORIES: GEO

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