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RNA-Seq analysis of differentially expressed genes in UPM-exposed epithelium co-cultivated with macrophages and dendritic cells from patients with asthma and COPD

ABSTRACT: The study aimed at evaluation and comparison of airway epithelium mRNA profile in response to airborne particulate matter presented by macrophages using in vitro triple cell co-culture model.

ORGANISM(S): Homo sapiens

PROVIDER: GSE175541 | GEO | 2022/08/16

REPOSITORIES: GEO

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Integrated Genomics Approaches Identify Mediators of Transcriptional and Epigenetic Responses to Afghan Desert Particulate Matter in Small Airway Epithelial Cells (PRO-Seq)

Project description:New respiratory diseases in personnel deployed to Southwest Asia after September 11th raise major concerns about the impacts of airborne particulate matter. Although regulations exist, the knowledge about how particulates influence disease states is limited, precluding the appropriate recognition, prevention and treatment of deployment-related lung diseases. We applied two genomics assays, Precision Run-on sequencing (PRO-seq) and the assay for transposase accessible chromatin with sequencing (ATAC-seq), to characterize the small airway epithelial cell response to Afghan desert particulate matter (APM).

2022-04-27 | GSE201150 | GEO

Integrated Genomics Approaches Identify Mediators of Transcriptional and Epigenetic Responses to Afghan Desert Particulate Matter in Small Airway Epithelial Cells (ATAC-Seq)

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Ambient Pollution Related Reprogramming of the Human Small Airway Epithelial Transcriptome

Project description:Epidemiological studies have demonstrated that exposure to particulate matter (PM) ambient pollution has adverse effects on lung health, exacerbated by cigarette smoking. Fine airborne particles <2.5 µm (PM2.5) are the most harmful of the urban pollutants, and the most closely linked to respiratory disease. Based on the knowledge that the small airway epithelium (SAE) plays a central role in pathogenesis of smoking-related lung disease, we hypothesized that elevated PM2.5 levels are associated with dysregulation of SAE gene expression.

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Project description:Healthy human subjects were exposed to wood smoke particulate matter and filtered air on separate occasions. Alveolar leukocytes (predominantly macrophages) were then obtained and analysed by microarray to assess the impact of wood smoke on transcription in the airway.

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Wistar rat model of COPD: Cigarette smoking exposure and fine particulate matter (PM2.5) exposure vs. control.

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Airborne particulate matter from goat farm increases acute allergic airway responses in mice increases acute allergic airway responses in mice

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