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Comparative Transcriptomics on cellular reactions to short-term hypergravity [Microarray]

ABSTRACT: Jurkat T cells have been lysed after exposure to 15 minutes of 9g in a ground-based pipette centrifuge. The RNA samples were parallely analyzed with RNA-Seq and microarray transcriptomics.

ORGANISM(S): Homo sapiens

PROVIDER: GSE175798 | GEO | 2021/08/31

REPOSITORIES: GEO

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Comparative Transcriptomics on cellular reactions to short-term hypergravity [RNA-Seq]

Project description:Jurkat T cells have been lysed after exposure to 15 minutes of 9g in a ground-based pipette centrifuge. The RNA samples were parallely analyzed with RNA-Seq and microarray transcriptomics.

2021-08-31 | GSE175799 | GEO

Jurkat T cell exposure to short hypergravity on a ground-based facility

Project description:Jurkat T cells have been lysed after exposure to 3 or 15 minutes of 9g hypergravity in a ground-based pipette centrifuge. The RNA samples were analyzed with RNA-Seq transcriptomics.

2022-07-27 | GSE190484 | GEO

Short-term hypergravity effects on Jurkat T cells

Project description:Jurkat T cells have been exposed to 9g hypergravity in a custom-built pipette centrifuge for different times (GBF2021). Additionally, Jurkat T cells have been exposed to 300g for 5 minutes in a standard benchtop centrifuge, and waited for different times until adding lysis buffer. For comparison, Jurkat T cells have been exposed to 5 minutes of 42°C.

2023-02-07 | GSE221579 | GEO

Comparative Transcriptomics on cellular reactions to short-term hypergravity

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-08-31 | GSE175800 | GEO

Plasma from Rainbow trout housed for 7 days in normoxic and hypoxic water

Project description:Hypoxic water had 5 mg/L of Dissolved Oxygen (DO) and normoxic water had 11.5 mg/L DO. 40 immature rainbow trout (about 100 g) were housed for 7 days under these conditions (20 fish in each treatment). Then fish were anesthetized with 100 mg/L MS-222, and blood was sampled using caudal puncture with heparinized syringes. The blood was centrifuged at 10 000 x g for 4 minutes in Heraeus Fresco 21 centrifuge (Thermo Fisher). Plasma was removed from this using a pipette, and placed in cryotubes, flash frozen in liquid nitrogen, and then stored at -80oC until preparation for proteomic analysis.

2021-06-18 | PXD026589 | Pride

Comparative Transcriptomics on cellular reactions to short-term hypergravity

Project description:Comparative Transcriptomics on cellular reactions to short-term hypergravity

| PRJNA733765 | ENA

Comparative Transcriptomics on cellular reactions to short-term hypergravity [Microarray]

Project description:Comparative Transcriptomics on cellular reactions to short-term hypergravity [Microarray]

| PRJNA733763 | ENA

Comparative Transcriptomics on cellular reactions to short-term hypergravity [RNA-Seq]

Project description:Comparative Transcriptomics on cellular reactions to short-term hypergravity [RNA-Seq]

| PRJNA733767 | ENA

Chromosome conformation capture of Jurkat T cells during parabolic flight

Project description:Jurkat T cells have been fixated at different gravity conditions during the 4th Swiss Parabolic Flight Campaign (4SPFC). 1g inflight samples were generated by crosslinking 5 minutes prior to onset of the first parabola. Hypg samples were generated by crosslinking the samples at the end of the first hypergravity 1.8 g phase, 20 seconds after start of the parabola. µg/0g samples were generated by crosslinking the cells 20 seconds after the onset of microgravity during the first parabola. A ground control reference condition was generated by crosslinking cells that were stored inside the flight hardware but were not onboard during the flight.

2024-05-01 | GSE174291 | GEO

Gene expression response to simulated gravity and hypergravity in human T cells

Project description:We investigated differentially regulated and stably expressed genes in human Jurkat T lymphocytic cells in 5min simulated microgravity and hypergravity and compared expression profiles to identify gravity-regulated and unaffected genes as well as adaptation processes.

2018-03-08 | GSE101101 | GEO

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