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M. tuberculosis transcriptional profile of delta pepD strain vs wild-type strain

ABSTRACT: pepD is regulated by a 2CS, MprAB, and impacts upon the regulation of sigE, an important M.tb sigma factor. Here we examine the global impact of gene regulation in a delta pepD mutant strain.

ORGANISM(S): Mycobacterium tuberculosis

PROVIDER: GSE17587 | GEO | 2009/10/30

SECONDARY ACCESSION(S): PRJNA118519

REPOSITORIES: GEO

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M. tuberculosis transcriptional profile of delta pepD strain vs wild-type strain

Project description:pepD is regulated by a 2CS, MprAB, and impacts upon the regulation of sigE, an important M.tb sigma factor. Here we examine the global impact of gene regulation in a delta pepD mutant strain. Analysis compares total RNA from early log growth cultures of both wild-type and a delta pepD strain.

2009-10-29 | E-GEOD-17587 | biostudies-arrayexpress

Sigma factor E of Mycobacterium tuberculosis controls the expression of bacterial components that modulate macrophages

2008-03-27 | GSE7962 | GEO

Wild-type yeast vs delta esl1/ delta esl2 mutant strain

Project description:Transcript profiling of the double delta esl1/ delta esl2 mutant versus wild-type growning at log phase in rich meda. During its natural life cycle, budding yeast (Saccharomyces cerevisiae) has to adapt to drastically changing environments, but how environmental sensing pathways are linked to adaptive gene expression changes remains incompletely understood. Here, we describe two closely related yeast hEST1A-B (SMG5-6)-like proteins termed Esl1 and Esl2 that contain a 14-3-3-like domain and a putative PIN ribonuclease domain. We found that, unlike their metazoan orthologues, Esl1/2 were not involved in nonsense-mediated mRNA decay or telomere maintenance pathways. However, in genome-wide expression array analyses, absence of Esl1 and Esl2 led to >2-fold deregulation of ~50 transcripts, most of which were expressed inversely to the appropriate metabolic response to environmental nutrient supply; for instance, normally glucose-repressed genes were de-repressed in esl1Δ esl2Δ double mutants during growth in a high-glucose environment. Likewise, in a genome-wide synthetic gene array screen, esl1Δ esl2Δ double mutants were synthetic sick with null mutations for Rim8 and Dfg16, which form the environmental sensing complex of the Rim101 pH response gene expression pathway. Overall, these results suggest that Esl1 and Esl2 contribute to the regulation of adaptive gene expression responses of environmental sensing pathways.

2013-07-18 | GSE48956 | GEO

rrp6 delta and rrp6 delta, trf4 delta vs WT

Project description:BMA64 rrp6 delta::HIS3 vs BMA64 WT (Mat a), grown at 30°C to OD 0.6 in YPD. BMA64 rrp6 delta::URA3, trf4 delta::KAN vs BMA64 WT, grown at 30°C to OD<0.6 in YPD. Keywords: other

2005-04-28 | GSE2579 | GEO

The sigma factor E of Mycobacterium tuberculosis modulates the inflammatory response of macrophages

Project description:Mycobacterium tuberculosis (Mtb) is well adapted to survive in macrophages and usually subverts the bactericidal mechanisms of these professional phagocytes. The adaptation of Mtb to the intracellular life depends on its ability to regulate the expression of its genes. Among the most important bacterial transcription activators are the sigma factors that bind to the RNA polymerase and give it promotor specificity. Sigma factor E (SigE) controls the expression of genes that are essential for Mtb virulence. Analysis of the macrophage transcriptional response indicated that proteins encoded by the sigE regulon are involved in the modulation of the macrophage inflammatory response. Keywords: Comparison of responses to infections

2008-03-27 | GSE7871 | GEO

The sigma factor E of Mycobacterium tuberculosis modulates the inflammatory response of human THP1 cells

2008-03-27 | GSE7870 | GEO

The sigma factor E of Mycobacterium tuberculosis modulates the inflammatory response of macrophages

2010-05-25 | E-GEOD-7871 | biostudies-arrayexpress

The Mycobacterium tuberculosis ECF sigma Factor sE: role in global gene expression and survival in macrophages

Project description:In previously published work, we identified three Mycobacterium tuberculosis sigma (s) factor genes responding to heat shock (sigB, sigE and sigH ). Two of them (sigB and sigE ) also responded to SDS exposure. As these responses to stress suggested that the s factors encoded by these genes could be involved in pathogenicity, we are studying their role in physiology and virulence. In this work, we characterize a sigE mutant of M. tuberculosis H37Rv. The sigE mutant strain was more sensitive than the wild-type strain to heat shock, SDS and various oxidative stresses. It was also defective in the ability to grow inside both human and murine unactivated macrophages and was more sensitive than the wild-type strain to the killing activity of activated murine macrophages. Using microarray technology and quantitative reverse transcriptionÐpolymerase chain reaction (RTÐPCR), we started to define the sigmaE regulon of M. tuberculosis and its involvement in the global regulation of the stress induced by SDS. We showed the requirement for a functional sigE gene for full expression of sigB and for its induction after SDS exposure but not after heat shock. We also identified several genes that are no longer induced when sigmaE is absent. These genes encode proteins belonging to different classes including transcriptional regulators, enzymes involved in fatty acid degradation and classical heat shock proteins. Keywords: genetic modification design and comparative genome hybridization design

2007-08-03 | GSE8664 | GEO

The sigma factor E of Mycobacterium tuberculosis modulates the inflammatory response of human THP1 cells

2010-05-25 | E-GEOD-7870 | biostudies-arrayexpress

Sigma factor E of Mycobacterium tuberculosis controls the expression of bacterial components that modulate macrophages

Project description:Sigma factor E (SigE) controls the expression of genes that are essential for Mtb virulence. In this work, we have identified the SigE regulon during infection of macrophages Our results indicate that SigE regulates the expression of genes involved in the maintenance of Mtb cell envelope integrity and function (i.e., detoxification and secretion). Keywords: strains comparison We compared the global gene expression of the H37Rv strain and the sigE mutant strain of Mtb in different conditions: growing exponentially in liquid media; after 2h of incubation in RPMI; or after 24 hours of infection of human macrophage-like THP-1 cells.

2010-05-25 | E-GEOD-7962 | biostudies-arrayexpress

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