NicE-C efficiently reveals open chromatin associated chromosome interactions at high resolution

ABSTRACT: Enhancer-promoter communication is known to regulate spatiotemporal gene expression. Here, we present NicE-C, a method that leverages nicking enzyme mediated open chromatin profiling and chromosome conformation capture to enable robust and cost-effective detection of chromatin interactions among open chromatin regions (e.g., between enhancers and promoters). Demonstrating its application utility, NicE-C revealed dynamic enhancer-promoter interactions associated with gene expression changes after TNFɑ stimulation in HeLa cells and during aging process in mice kidney.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE176066 | GEO | 2022/01/18

REPOSITORIES: GEO