Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Experiment 2: Pou5f1 and Sox2 overexpression experiments with or without protein synthesis inhibition

ABSTRACT: Pou5f1 and Sox2 overexpression experiments with and without protein synthesis inhibitor were performed to investigate direct transcriptional targets of Pou5f1 and Sox2

ORGANISM(S): Danio rerio

PROVIDER: GSE17659 | GEO | 2010/03/01

SECONDARY ACCESSION(S): PRJNA123381

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Experiment 2: Pou5f1 and Sox2 overexpression experiments protein synthesis inhibition

Project description:Pou5f1 and Sox2 overexpression experiments with protein synthesis inhibitor were performed to investigate direct transcriptional targets of Pou5f1 and Sox2

2013-07-31 | GSE41107 | GEO

Experiment 2: Pou5f1 and Sox2 overexpression experiments protein synthesis inhibition

Project description:Pou5f1 and Sox2 overexpression experiments with protein synthesis inhibitor were performed to investigate direct transcriptional targets of Pou5f1 and Sox2 Experiments were performed in biological triplicates. Cy3 and Cy5 channels were split and analyzed separately using Genedata Analyst software, quantile normalised and used for independent comparisons.

2013-07-31 | E-GEOD-41107 | biostudies-arrayexpress

Zygotic gene activatory network in zebrafish is globally controlled by Pou5f1 SoxB1 enhancers

Project description:Genetic control of pluripotent mammalian ES cells is determined by a transcriptional network, with a "central core" of transcription factors, Pou5f1, Sox2 and Nanog. Zebrafish homologues of the "core pluripotency factors" Pou5f1, SoxB1 and Nanog-like are also crucially involved in early development. However, the degree of functional similarity of the network between mammals and non-mammals is a matter of debate. To identify the components of Pou5f1-dependent transcriptional networks, we determined the genomic binding sites for Pou5f1 and Sox2 in late blastula stage zebrafish embryos using ChIP-seq. We found that Sox2 and Pou5f1 are co-binding to the regulatory regions of Sox2, Pou5f1, and Nanog-like, as well as to multiple orthologues of mammalian plutipotency network components.

2013-08-10 | GSE39780 | GEO

Zygotic gene activatory network in zebrafish is globally controlled by Pou5f1 SoxB1 enhancers

2013-08-10 | E-GEOD-39780 | biostudies-arrayexpress

Experiment 1: Overexpression experiments at 30% epiboly MZspg and WT embryos with or without protein synthesis inhibition

2013-12-31 | GSE44982 | GEO

Experiment 1: Overexpression experiments at 30% epiboly MZspg and WT embryos with or without protein synthesis inhibition

Project description:Overexpression experiments with or without protein synthesis inhibition were performed to investigate transcriptional targets of Klf4, FoxD3, and Nanog-like in the blastula stage embryos (4.7 hours postfertilization) of wild type or MZspg background. Experiments were performed in biological triplicates. Cy3 and Cy5 channels were splitted in Genedata Expressionist and Analyst software, quantile normalised and used for independent comparisons.

2013-12-31 | E-GEOD-44982 | biostudies-arrayexpress

Experiment 2: Klf2a, Klf2b and FoxD3 overexpression experiments at 30% epiboly MZspg and WT embryos with or without protein synthesis inhibition

2013-12-31 | GSE45010 | GEO

Experiment 2: Klf2a, Klf2b and FoxD3 overexpression experiments at 30% epiboly MZspg and WT embryos with or without protein synthesis inhibition

Project description:Overexpression experiments with or without protein synthesis inhibition were performed to investigate transcriptional targets of Klf2a, Klf2b and FoxD3 in the blastula stage embryos (4,7 hours postfertilization) of wild type or MZspg backgroumd Experiments were performed in biological duplicates. Cy3 and Cy5 channels were splitted in Genedata Analyst software, quantile normalised and used for independent comparisons.

2013-12-31 | E-GEOD-45010 | biostudies-arrayexpress

Identification the Expression of Sox2 in Colorectal Cancer Tissues

Project description:Sox2 has been studied in several types of human solid tumors. The investigators found that Sox2 had higher expression level in colorectal cancer and metastatic tissues than normal tissues. So the investigators assumed that whether Sox2 plays an important role in the progression and migration of colon cancer.

| 2124687 | ecrin-mdr-crc

POU5F1 induces cellular heterogeneity in colorectal cancer

Project description:In this study, we aimed to identify CRC treatment resistance mechanisms by focusing on POU5F1-positive cells. Despite the small number of POU5F1-positive cells, single POU5F1-expressing cells produced heterogeneous cell populations in vitro and in vivo colorectal cancer organod. This study unveils a mechanism of CRC treatment resistance and highlights the potential of POU5F1-expressing cells as CTCs.

2023-11-29 | GSE169220 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data