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The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations

ABSTRACT: The gene expression profile of E. coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole genome oligonucleotide microarrays. “Pool” RNA was isolated from five independently grown 50ml DMA cultures of E. coli K-12 MG1655. “Test” RNA was isolated from three independently grown 50ml DMA cultures of E. coli K-12 MG1655, for each growth condition (no added CuSO4, or supplemented with either 0.75mM or 2mM copper glycine). A sample of each culture was taken at mid to late exponential growth phase at OD600 0.8 (measured using a Pharmacia Biotech Ultrospec 2000 in a cuvette with a path length of 10mm). Keywords: dose response

ORGANISM(S): Escherichia coli

PROVIDER: GSE1780 | GEO | 2004/09/22

SECONDARY ACCESSION(S): PRJNA90573

REPOSITORIES: GEO

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The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations

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