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Inducible degradation of the Mediator subunit Med19 reveals a dedicated role in the regulation of developmental genes expression

ABSTRACT: In the Drosophila wing imaginal disc, by using a inductible degradable version of Med19 degradation with RNAseq, we got access to the early consequences of Med19 depletion on gene expression. We showed that less than a quarter of the genes expressed in the wing disc were deregulated after Med19 loss. Strikingly, these genes exhibit a spatio-temporal expression pattern like transcription factors or Notch responsive genes that reveals Med19 dedicated role in the regulation of developmental gene transcription.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE178125 | GEO | 2023/12/31

REPOSITORIES: GEO

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Drosophila melanogaster

Project description:Wing imaginal disc spatial expression

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Whole animal vs. tissues at 18h before puparium formation

Project description:Comparisons between whole animal and epidermis with attached muscle, salivary gland, wing disc, midgut, and central nervous system tissues at approximately 18 hours before pupariation. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development Keywords: other

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RNA-seq of regenerating blastema cells in Drosophila melanogaster

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18h before puparium formation vs. puparium formation tissue comparisons

Project description:Samples at 18h before puparium formation were compared to samples at puparium formation for each the following tissues: epidermis with attached muscle, salivary gland, wing disc, midgut, and central nervous system. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development Keywords: other

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18h before puparium formation vs. puparium formation tissue comparisons

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Gene expression changes in Nicd induced wing disc hyperplasia

Project description:The outcome of Notch proliferation on proliferation depends on the context. In Drosophila wing imaginal discs Notch activation causes hyperplasia despite having localized inhibitory effects on proliferation. To understand te underlying mechanisms we have used genomic strategies to identify the Notch-Su(H) target genes during wing discs hyperplasis. these data are the results from expression profiling the RNAs from hyperplastic wing discs overexpressing Nicd. Direct comparison of third instar lavae wing imaginal disc Nicd (abxUbxFLPase; Act>y>Gal4, UAS GFP; FRT82B tubGal80 with UAS-Nicd; FRT82B) vs control (abxUbxFLPase; Act>y>Gal4, UAS GFP; FRT82B tubGal80 with FRT82B ). 4 Biological replicates, the 2nd replicate was performed as a dye-swap.

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Gene expression changes in Su(H) induced wing disc hyperplasia

Project description:The outcome of Notch activation of proliferation depends on cellular context. In Drosophila wing discs Notch pathway overactivation results in hyperplasia. To understand the mechanisms we have used genomic strategies to indetify the Notch-S(H) target genes directly regulated in wing disc hyperplasia. These data are the results from expression profiling the RNAs from hyperplastic wing discs overexpressing Su(H). Direct comparison of Giant third instar lavae wing imaginal disc (UAS-GFP:Su(H) expressed by the patched[559.1]-Gal4 driver) vs control (UAS-NLS-GFP expressed by the patched[559.1]-Gal4 driver). 3 Biological replicates, the 3rd replicate was performed as a dye-swap.

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Genome-wide maps of the androgen receptor and H3K27 upon MED19 overexpression in LNCaP cells

Project description:We report the application of ChIP and RNA sequencing to identify the mechanism whereby stable overexpression of MED19 in androgen-dependent LNCaP cells promotes growth under conditions of androgen deprivation. We determined the MED19 and AR transcriptomes and cistromes in control and MED19 LNCaP cells. We also examined genome-wide H3K27 acetylation in both the absence and presence of androgens. We found that MED19 overexpression selectively alters AR occupancy, H3K27 acetylation, and gene expression. Under conditions of androgen deprivation, genes regulated by MED19 correspond to genes regulated by ELK1, a transcription factor that binds the AR N-terminus to induce select AR target gene expression and proliferation. This study provides important insight into the mechanisms of prostate cancer cell growth under low androgen, and underscores the importance of the MED19 in this process.

2021-01-20 | GSE161167 | GEO

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