Dataset Information


Gene expression profile of effector Treg cells isolated from spleens and tumors of tumor-bearing mice

ABSTRACT: A majority of effector Treg cells (eTregs) that are abundant in many types of tumors are marked by the expression of Blimp1. However, the specific impact of Blimp1+ eTregs on, and mechanisms of action within, tumors remain unknown. To better understand the role of Blimp1 in the regulation of eTreg function in the tumor immunity, we profiled the differential gene expression in Blimp1-deficient eTregs compared to WT control eTregs from tumor-bearing mice. Overall design: Prdm1fl/flFoxP3YFP-Cre (KO) and FoxP3YFP-Cre (WT) mice were subcutaneously inoculated with B16.OVA at day 0, and intraperitoneally immunized with NP-OVA in CFA at day 0 and NP-OVA in IFA at day 7. When the tumor reached ≤ 1.5 cm3, CD45+CD44+YFP+(FoxP3+)CD4+CD3+ Tregs were FACS-sorted from spleens and tumors of these mice. RNA was prepared and sequencing was performed at Genewiz (South Plainfield, NJ) using Ultra-Low Input RNA-Seq Service.

INSTRUMENT(S): Illumina HiSeq 4000 (Mus musculus)

ORGANISM(S): Mus musculus  

SUBMITTER: Jianmei Wu Leavenworth  

PROVIDER: GSE178132 | GEO | 2021-11-24


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Remodeling of the tumor microenvironment via disrupting Blimp1<sup>+</sup> effector Treg activity augments response to anti-PD-1 blockade.

Dixon Michael L ML   Luo Lin L   Ghosh Sadashib S   Grimes Jeffrey M JM   Leavenworth Jonathan D JD   Leavenworth Jianmei W JW  

Molecular cancer 20211120 1

<h4>Background</h4>Accumulation of Foxp3<sup>+</sup> regulatory T (Treg) cells in the tumor often represents an important mechanism for cancer immune evasion and a critical barrier to anti-tumor immunity and immunotherapy. Many tumor-infiltrating Treg cells display an activated phenotype and express the transcription factor Blimp1. However, the specific impact of these Blimp1<sup>+</sup> Treg cells and their follicular regulatory T (T<sub>FR</sub>) cell subset on tumor and the underlying mechani  ...[more]

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