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Functional genomics of mountain pine beetle (Dendroctonus ponderosae) midguts and fatbodies

ABSTRACT: Survey of relative gene expression profiles in larvae, pupae, and midguts and fatbodies of teneral, unfed, and fed adult mountain pine beetles from western Nevada, USA.

ORGANISM(S): Dendroctonus ponderosae

PROVIDER: GSE17858 | GEO | 2009/08/29

SECONDARY ACCESSION(S): PRJNA120101

REPOSITORIES: GEO

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Functional genomics of mountain pine beetle (Dendroctonus ponderosae) midguts and fatbodies

Project description:Survey of relative gene expression profiles in larvae, pupae, and midguts and fatbodies of teneral, unfed, and fed adult mountain pine beetles from western Nevada, USA. Array prepared on 4-tile chips (4-plex custom arrays), each feature represented by six different oligonucleotides, three replicate blocks/tile, hybridized with total RNA from 11 samples, each replicated four times. Vector (pDONR222) and two plant sequences included as negative controls.

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Quantitative transcriptomics of pheromone biosynthetic midgut and fat body tissues in the mountain pine beetle, Dendroctonus ponderoase Hopkins

Project description:Bark beetles (Coleoptera: Scolytinae) are pests of many forests around the world. The mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins, is a significant pest of western North American pine forests. The MPB is able to overcome the defences of pine trees through pheromone-assisted aggregation that results in a mass attack of host trees. These pheromones, both male and female produced, are believed to be biosynthesized in the midgut and/or fat body of these insects. We have used transcriptomics (RNA-seq) to identify transcripts differentially expressed between sexes and between tissues, with juvenile hormone III treatment, which is known to induce pheromone biosynthesis.

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Functional genomics of mountain pine beetle (Dendroctonus ponderosae) midguts and fat bodies.