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DLEU1 promotes oral squamous cell carcinoma progression through activating interferon-stimulated genes [microarray]

ABSTRACT: Long noncoding RNAs (lncRNAs) are deeply involved in cancer development, and we have previously reported that DLEU1 (deleted in lymphocytic leukemia 1) is frequently overexpressed in oral squamous cell carcinoma (OSCC) cells, where it plays an oncogenic role. In this study, we aimed to further clarify the molecular function of DLEU1 in the pathogenesis of OSCC. Chromatin immunoprecipitation-sequencing (ChIP-seq) analysis revealed that knockdown of DLEU1 induced substantial changes in the levels of histone H3 lysine 4 trimethylation (H3K4me3) and H3K27 acetylation (H3K27ac) in OSCC cells. Notably, depletion of DLEU1 significantly downregulated levels of H3K4me3/H3K27ac and expression of a number of interferon-stimulated genes (ISGs) including IFIT1, IFI6 and OAS1, while ectopic DLEU1 expression activated these genes. Western blot analysis and reporter assay suggested that DLEU1 upregulates ISGs through activating the JAK-STAT signaling in OSCC cells. Moreover, IFITM1, one of the ISGs induced by DLUE1, is frequently overexpressed in primary OSCC tumors, and knockdown of IFITM1 attenuated OSCC cell proliferation, migration and invasion. Our data suggest that DLEU1 exerts its oncogenic function through, at least in part, activating a series ISGs in OSCC cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE178608 | GEO | 2021/09/12

REPOSITORIES: GEO

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DLEU1 promotes oral squamous cell carcinoma progression through activating interferon-stimulated genes [ChIP-Seq]

Project description:Long noncoding RNAs (lncRNAs) are deeply involved in cancer development, and we have previously reported that DLEU1 (deleted in lymphocytic leukemia 1) is frequently overexpressed in oral squamous cell carcinoma (OSCC) cells, where it plays an oncogenic role. In this study, we aimed to further clarify the molecular function of DLEU1 in the pathogenesis of OSCC. Chromatin immunoprecipitation-sequencing (ChIP-seq) analysis revealed that knockdown of DLEU1 induced substantial changes in the levels of histone H3 lysine 4 trimethylation (H3K4me3) and H3K27 acetylation (H3K27ac) in OSCC cells. Notably, depletion of DLEU1 significantly downregulated levels of H3K4me3/H3K27ac and expression of a number of interferon-stimulated genes (ISGs) including IFIT1, IFI6 and OAS1, while ectopic DLEU1 expression activated these genes. Western blot analysis and reporter assay suggested that DLEU1 upregulates ISGs through activating the JAK-STAT signaling in OSCC cells. Moreover, IFITM1, one of the ISGs induced by DLUE1, is frequently overexpressed in primary OSCC tumors, and knockdown of IFITM1 attenuated OSCC cell proliferation, migration and invasion. Our data suggest that DLEU1 exerts its oncogenic function through, at least in part, activating a series ISGs in OSCC cells.

2021-09-12 | GSE178611 | GEO

Effects of DLEU1 knockdown on gene expression profiles in oral squamous cell carcinoma cells

Project description:We identified DLEU1 as a long noncoding RNA (lncRNA) frequently overexpressed in oral squamous cell carcinoma (OSCC). Knockdown of DLEU1 suppressed proliferation, migration and invasion and induced cell cycle arrest and apoptosis in OSCC cells. To further clarify the molecular function of DLEU1, we carried out gene expression microarray analysis in OSCC cell lines with DLEU1 knockdown. We found that genes associated with “matrix metalloproteinases”, “apoptosis”, “cell cycle” and “MAPK signaling” were enriched among the affected genes. These results suggest that DLEU1 may play a role in the development of OSCC.

2018-09-23 | GSE108851 | GEO

Gene expression data in DLEU1 downregulated BL cell

Project description:Comparison of gene expression profiling between DLEU1 downregulated Raji BL cell and wild type Raji BL cell Transcription Activator-Like Effector Nucleases (TALENs) mediated DLEU1 downregulated Raji burkitt lymphoma (BL) cell clone and wild type (control) Raji cell clone were analyzed in duplicate. Four samples were hybridized to HGU133_plus_2 Genechip(Affymetrix) and scanned with the Scanner 3000 7G.

2015-06-04 | E-GEOD-65674 | biostudies-arrayexpress

Gene expression data in DLEU1 downregulated BL cell

Project description:Comparison of gene expression profiling between DLEU1 downregulated Raji BL cell and wild type Raji BL cell

2015-06-04 | GSE65674 | GEO

DLEU1 promotes oral squamous cell carcinoma progression through activating interferon-stimulated genes

Project description:DLEU1 promotes oral squamous cell carcinoma progression through activating interferon-stimulated genes

| PRJNA739720 | ENA

DLEU1 promotes oral squamous cell carcinoma progression through activating interferon-stimulated genes [microarray]

Project description:DLEU1 promotes oral squamous cell carcinoma progression through activating interferon-stimulated genes [microarray]

| PRJNA739714 | ENA

DLEU1 promotes oral squamous cell carcinoma progression through activating interferon-stimulated genes [ChIP-Seq]

Project description:DLEU1 promotes oral squamous cell carcinoma progression through activating interferon-stimulated genes [ChIP-Seq]

| PRJNA739721 | ENA

The function and mechanism about how ORAOV1-B potentiates the OSCC metastasis.

Project description:Lymph node metastasis, a powerful prognostic indicator of oral squamous cell carcinoma (OSCC), is chiefly responsible for cancer death. Long non-coding RNA (lncRNA) is recently addressed to significantly account for modulating OSCC metastasis. Here, we identified a novel alternative splice of Oral Cancer Overexpressed 1 (ORAOV1), ORAOV1-B, which was subsequently validated as an lncRNA and correlated with OSCC lymph node metastasis; significantly increased invasion and migration were observed in ORAOV1-B-overexpressed OSCC cells; In the metastatic model, ORAOV1-B also significantly contributed to OSCC-related lung metastasis. cDNA microarrays was performed to compare up- or down-regulated genes in EV and ORAOV1-B sets of OSCC, which suggested TNFα as a potential downstream of ORAOV1-B and the upregulation of pro-inflammatory genes, indicating the activation of NF-κB pathway. In summary, the novel splice variant ORAOV1-B is an lncRNA, which significantly potentiates OSCC invasion and metastasis by binding to Hsp90 and activating the NF-κB-TNFα loop. Our study implies that ORAOV1-B might serve as an attractive OSCC metastasis intervention target.

2020-03-04 | GSE145599 | GEO

PRDM16 binding in brown adipocyte precursors [ChIP-seq]

Project description:PRDM16 is an important transcriptional regulator of brown and beige adipocyte gene programs. We discovered that PRDM16 also represses type I Interferon-stimulated genes (ISGs) in brown and beige adipocytes. ChIP-seq demonstrated that PRDM16 binds proximal to many of the regulated ISGs and this correlates with reduction in the amount of the activating mark, H3K27Ac. Together this data shows PRDM16 represses ISG expression through direct binding at promoter regions to block transcription.

2017-04-10 | GSE86017 | GEO

Cellular transcriptomes of wild-type and ING3 knockout HT-29 cells

Project description:The goal of this study is to use RNA-sequencing to profile the transcriptional changes in HT-29 cells with ING3 deficiency. Specifically, we were interested in determining the IFN and ISGs in response to ING3 KO. Several ISGs including IFI44L and IFITM1 were up-regulated in ING3 KO HT-29 cells, indicating that the broad resistance to virus infection in ING3 KO cells were mediated by heightened IFN and ISG responses.

2021-08-04 | GSE166219 | GEO

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