Transcriptomics,Genomics

Dataset Information

19

Whole genome analysis of Sertoli cell gene expression by retinoblastoma-1


ABSTRACT: Analysis of the regulation of gene expression profiles by retinoblastoma-1 in Sertoli cells. Conditional knockout of Rb1 in Sertoli cells led to progressive infertiliy in male mice that occured between 10 and 14 weeks of age. Results of gene expression studies performed on 6 week-old purified Sertoli cells helped elucidate the key role of RB1 in mature, differentiated Sertoli cells. Overall design: Total RNA was obtained from 6 week-old Sox9-GFP labeled Sertoli cells that were fluorescently sorted (FACSAriaII cell sorter, BD)

INSTRUMENT(S): Illumina MouseWG-6 v2.0 expression beadchip

SUBMITTER: Martin M Matzuk   

PROVIDER: GSE17904 | GEO | 2009-09-02

SECONDARY ACCESSION(S): PRJNA119973

REPOSITORIES: GEO

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Publications

Retinoblastoma protein plays multiple essential roles in the terminal differentiation of Sertoli cells.

Nalam Roopa L RL   Andreu-Vieyra Claudia C   Braun Robert E RE   Akiyama Haruhiko H   Matzuk Martin M MM  

Molecular endocrinology (Baltimore, Md.) 20091009 11


Retinoblastoma protein (RB) plays crucial roles in cell cycle control and cellular differentiation. Specifically, RB impairs the G(1) to S phase transition by acting as a repressor of the E2F family of transcriptional activators while also contributing towards terminal differentiation by modulating the activity of tissue-specific transcription factors. To examine the role of RB in Sertoli cells, the androgen-dependent somatic support cell of the testis, we created a Sertoli cell-specific conditi  ...[more]

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