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Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ada2b]

ABSTRACT: Purpose: The goal of this study was to identify the differential expressed genes in the Drosophila embryo when ada2b is disrupted.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE179060 | GEO | 2021/12/21

REPOSITORIES: GEO

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Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos.

Project description:The histone acetyltransferase Gcn5 is critical for gene expression and development. In Drosophila, Gcn5 is part of four complexes (SAGA, ATAC, CHAT and ADA) that are essential for fly viability and have key roles in regulating gene expression. Here, we show that although the SAGA, ADA and CHAT complexes play redundant roles in embryonic gene expression, the insect-specific CHAT complex uniquely regulates expression of a subset of developmental genes. We also identify a substantial decrease in histone acetylation in chiffon mutant embryos that exceeds that observed in Ada2b, suggesting broader roles for Chiffon in regulating histone acetylation outside of the Gcn5 complexes. The chiffon gene encodes two independent polypeptides that nucleate formation of either the CHAT or Dbf4-dependent kinase (DDK) complexes. DDK includes the cell cycle kinase Cdc7, which is necessary for maternally driven DNA replication in the embryo. We identify a temporal switch between the expression of these chiffon gene products during a short window during the early nuclear cycles in embryos that correlates with the onset of zygotic genome activation, suggesting a potential role for CHAT in this process. This article has an associated First Person interview with the first author of the paper.

| S-EPMC8917357 | biostudies-literature

Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ada2b]

Project description:Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ada2b]

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iChiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [chiffon]

Project description:Purpose: The goal of this study was to identify the differential expressed genes in the Drosophila embryo when chiffon is disrupted.

2021-12-21 | GSE179061 | GEO

Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-12-21 | GSE179065 | GEO

Drosophila Ada2b is required for viability and normal histone H3 acetylation.

Project description:Regulation of chromatin through histone acetylation is an important step in gene expression. The Gcn5 histone acetyltransferase is part of protein complexes, e.g., the SAGA complex, that interact with transcriptional activators, targeting the enzyme to specific promoters and assisting in recruitment of the basal RNA polymerase transcription machinery. The Ada2 protein directly binds to Gcn5 and stimulates its catalytic activity. Drosophila contains two Ada2 proteins, Drosophila Ada2a (dAda2a) and dAda2b. We have generated flies that lack dAda2b, which is part of a Drosophila SAGA-like complex. dAda2b is required for viability in Drosophila, and its deletion causes a reduction in histone H3 acetylation. A global hypoacetylation of chromatin was detected on polytene chromosomes in dAda2b mutants. This indicates that the dGcn5-dAda2b complex could have functions in addition to assisting in transcriptional activation through gene-specific acetylation. Although the Drosophila p53 protein was previously shown to interact with the SAGA-like complex in vitro, we find that p53 induction of reaper gene expression occurs normally in dAda2b mutants. Moreover, dAda2b mutant animals show excessive p53-dependent apoptosis in response to gamma radiation. Based on this result, we speculate that dAda2b may be necessary for efficient DNA repair or generation of a DNA damage signal. This could be an evolutionarily conserved function, since a yeast ada2 mutant is also sensitive to a genotoxic agent.

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Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ChIP-seq]

Project description:Purpose: The goal of this study was to identify H3K14ac levels in chiffon mutant vs WT

2021-12-21 | GSE179062 | GEO

Ada2b isoforms

Project description:Gene expression profiling of the two ADA2b protein (ADA2bS and ADA2bL) in Drosophila melanogaster

2012-09-30 | E-MEXP-3747 | biostudies-arrayexpress

Transcription profiling of Drosophila wild type and Ada2b knock out pupa

Project description:The study of the role of Drosophila Ada2b SAGA histone acetyltransferase component at early pupal stage (P4)

2009-04-25 | E-MEXP-2126 | biostudies-arrayexpress

Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos

Project description:Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos