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Single cell RNA sequencing of healthy donor human CAR-T cells and adjacent tumor tissue from NSG mice bearing ASPC-1 tumors that are treated with CAR-T cells

ABSTRACT: The goal of this study was to assess the consequences of RN7SL1 production on CAR-T cell gene expression cells within the ASCP-1 tumor microenvironment. RN7SL1 RNA-producing CAR-T cells are the relevant experimental condition, while control M5BBz CAR-T cells are also collected.

ORGANISM(S): Homo sapiens

PROVIDER: GSE179249 | GEO | 2021/09/01

REPOSITORIES: GEO

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Single cell RNA sequencing of healthy donor human CAR-T cells and adjacent tumor tissue from NSG mice bearing ASPC-1 tumors that are treated with CAR-T cells

Project description:Single cell RNA sequencing of healthy donor human CAR-T cells and adjacent tumor tissue from NSG mice bearing ASPC-1 tumors that are treated with CAR-T cells

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Project description:CEACAM5-specific CAR NK-92 cells were engineered to target CEACAM5-expressing lung tumor cells. To study their transcriptional response during tumor engagement, parental NK-92 cells, CEACAM5-CAR NK-92 cells, hydrocortisone-treated CAR NK-92 cells, NR3C1-knockout (cortisol-resistant) CAR NK-92 cells, and hydrocortisone-treated NR3C1-knockout CAR NK-92 cells were co-cultured with CEACAM5⁺ A549 lung cancer cells for 16 hours. Following co-culture, NK-92–derived effector cells were isolated by flow cytometry and processed for bulk RNA sequencing. This dataset captures transcriptional programs associated with CAR activation, hydrocortisone exposure, and glucocorticoid receptor deficiency in NK-92–based effector cells responding to CEACAM5⁺ tumor targets.

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