Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Time course of PBL derived avian macrophages stimulated with whole Ecoli or LPS

ABSTRACT: This series compares gene expression in chicken peripheral blood lymphocyte (PBL) derived macrophages following stimulation with either XL10 E. coli or S. typh LPS over an 8 hour time course (0,1,2,4,and 8 hours). For the late time points, the stimuli were removed after 2 hours and fresh media was added for the remaining time. Keywords: time-course

ORGANISM(S): Gallus gallus

PROVIDER: GSE1794 | GEO | 2004/09/24

SECONDARY ACCESSION(S): PRJNA90595

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Time course of PBL derived avian macrophages stimulated with whole Ecoli or LPS

Project description:This series compares gene expression in chicken peripheral blood lymphocyte (PBL) derived macrophages following stimulation with either XL10 E. coli or S. typh LPS over an 8 hour time course (0,1,2,4,and 8 hours). For the late time points, the stimuli were removed after 2 hours and fresh media was added for the remaining time.

2010-06-05 | E-GEOD-1794 | biostudies-arrayexpress

The Transcriptome of Utricle Hair Cell Regeneration in the Avian Inner Ear

Project description:A comprehensive transcriptome profile of chicken utricle hair cell across a 7 days regenerative time course.

2014-04-29 | GSE57134 | GEO

The Transcriptome of Basilar Papilla Hair Cell Regeneration in the Avian Inner Ear

Project description:A comprehensive transcriptome profile of chicken basilar papilla hair cell across a 7 days regenerative time course.

2015-02-21 | GSE66101 | GEO

SE challenged in 3 genetic chicken lines

Project description:3 genetic chicken lines (Leghorn G-B1, Fayoumi, broiler) were used. chicken were challenged with SE at day 1, spleen and cecum tissues were collected at 2 hours and 16 hours after post challenge Keywords: time-course

2006-04-23 | GSE2562 | GEO

The Transcriptome of Utricle Hair Cell Regeneration in the Avian Inner Ear

Project description:A comprehensive transcriptome profile of chicken utricle hair cell across a 7 days regenerative time course. Compare gene expression changes in chicken utricle treated with streptomycin to the control. A total of 60 samples were collected from 11 different time points. Each time point contains at least 2 biological samples.

2014-04-29 | E-GEOD-57134 | biostudies-arrayexpress

The Transcriptome of Basilar Papilla Hair Cell Regeneration in the Avian Inner Ear

Project description:A comprehensive transcriptome profile of chicken basilar papilla hair cell across a 7 days regenerative time course. Compare gene expression changes in chicken basilar papilla treated with streptomycin to the control. A total of 60 samples were collected from 8 different time points. Each time point contains 2 biological samples.

2015-02-21 | E-GEOD-66101 | biostudies-arrayexpress

Gene expression profiling of PBL in response to ionising radiation and modeled microgravity

Project description:BACKGROUND: Ionizing radiation (IR) can be extremely harmful for human cells since an improper DNA-damage response (DDR) to IR can contribute to carcinogenesis initiation. Perturbations in DDR pathway can originate from alteration in the functionality of the microRNA-mediated gene regulation, being microRNAs (miRNAs) small noncoding RNA that act as post-transcriptional regulators of gene expression. In this study we gained insight into the role of miRNAs in the regulation of DDR to IR under microgravity, a condition of weightlessness experienced by astronauts during space missions, which could have a synergistic action on cells, increasing the risk of radiation exposure. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed miRNA expression profile of human peripheral blood lymphocytes (PBL) incubated for 4 and 24 h in normal gravity (1 g) and in modeled microgravity (MMG) during the repair time after irradiation with 0.2 and 2Gy of γ-rays. Our results show that MMG alters miRNA expression signature of irradiated PBL by decreasing the number of radio-responsive miRNAs. Moreover, let-7i*, miR-7, miR-7-1*, miR-27a, miR-144, miR-200a, miR-598, miR-650 are deregulated by the combined action of radiation and MMG. Integrated analyses of miRNA and mRNA expression profiles, carried out on PBL of the same donors, identified significant miRNA-mRNA anti-correlations of DDR pathway. Gene Ontology analysis reports that the biological category of "Response to DNA damage" is enriched when PBL are incubated in 1 g but not in MMG. Moreover, some anti-correlated genes of p53-pathway show a different expression level between 1 g and MMG. Functional validation assays using luciferase reporter constructs confirmed miRNA-mRNA interactions derived from target prediction analyses. CONCLUSIONS/SIGNIFICANCE: On the whole, by integrating the transcriptome and microRNome, we provide evidence that modeled microgravity can affects the DNA-damage response to IR in human PBL.

2012-04-10 | GSE20173 | GEO

Effects of capture-release events in wild dolphins: PBL analysis

Project description:Goal: To determine the effects of capture-release events in wild dolphins (Tursiops truncatus). Methods: An analysis of the Peripheral Blood Leukocyte (PBL) transcriptome was conducted on a group of 20 animals. The samples were collected in 2 different locations along the US east coast (Charleston, SC; Indian River Lagoon, FL) and 2 blood samples were collected for each dolphin 1) immediately after the capture event (*pre*) and 2) just before the animal was released (*post*). In between *pre* and *post* blood collections (30-40 minutes) additional samples were collected from the animals for physiological, chemical and biochemical analysis. RNA extracted from *pre* and *post* blood samples was used for micorarray hybridizations and transcriptome analysis using a species-specific PBL cDNA microarray (Mancia *et al*., 2007). Keywords: blood cells (PBL)

2007-05-03 | GSE7691 | GEO

Conservation of the PBL-RBOH immune module in land plants

Project description:The rapid production of reactive oxygen species (ROS) is a key signaling output in plant immunity. In the angiosperm model species, Arabidopsis thaliana (hereafter Arabidopsis), recognition of non- or altered-self elicitor patterns by cell-surface immune receptors activates the receptor-like cytoplasmic kinases (RLCKs) of the AVRPPHB SUSCEPTIBLE 1 (PBS1)-like (PBL) family, particularly BOTRYTIS-INDUCED KINASE1 (BIK1)1–3. BIK1/PBLs in turn phosphorylate the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) to induce apoplastic ROS production4,5. PBL and RBOH functions in plant immunity have been extensively characterized in flowering plants. Much less is known about the conservation of pattern-triggered ROS signaling pathways in non-flowering plants. In this study, we show that, in the liverwort Marchantia polymorpha (hereafter Marchantia), single members of RBOH and PBL families, namely MpRBOH1 and MpPBLa, are required for chitin-induced ROS production. MpPBLa directly interacts with and phosphorylates MpRBOH1 at specific, conserved sites within its cytosolic N-terminus and this phosphorylation is essential for chitin-induced MpRBOH1-mediated ROS production. Collectively, our work reveals the functional conservation of the PBL-RBOH module that controls pattern-triggered ROS production in land plants.

2023-02-06 | PXD038639 | Pride

LPS Blood Time course

Project description:cDNA microarray study of gene expression changes in whole blood from LPS treated rats, 2 and 6 hours after I.P. injection of 5 mg/kg Keywords: time-course

2005-02-09 | GSE1658 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data