Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Nanostring nCounter Bovine Arrival BRD 60 Panel v1.0

ABSTRACT: The objective of this study was to corroborate the findings of previous BRD-associated transcriptome experiments, utilizing multiple independent populations of high-risk beef cattle.

ORGANISM(S): Bos taurus

PROVIDER: GSE179536 | GEO | 2022/03/02

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Nanostring nCounter Bovine Arrival BRD 60 Panel v1.0

Project description:Nanostring nCounter Bovine Arrival BRD 60 Panel v1.0

| PRJNA744165 | ENA

Longitudinal blood transcriptomic analysis to identify molecular regulatory patterns of Bovine Respiratory Disease in beef cattle

Project description:We profiled blood transcriptomics of 24 beef steers at three important stages (Entry: on arrival at the feedlot; Pulled: when sickness is identified; and Close-out: recovered, healthy cattle at shipping to slaughter) to reveal the key biological functions and regulatory factors of BRD and identify gene markers of BRD for early diagnosis and potentially use in selection.

2020-12-31 | GSE150706 | GEO

Applying multi-omics data to study the genetic background of bovine respiratory disease infection in feedlot crossbred cattle

Project description:Bovine respiratory disease (BRD) is the most common and costly infectious disease affecting the well-being and productivity of beef cattle in North America. BRD is a complex disease whose development is dependent on environmental factors and host genetics. Due to the polymicrobial nature of BRD, our understanding of the genetic and molecular mechanisms underlying the disease is still limited. This knowledge would augment the development of better genetic/genomic selection strategies and more accurate diagnostic tools to reduce BRD prevalence. Therefore, this study utilized multi-omics data (genomics, transcriptomics, and metabolomics) analyses to study the associations between genome, transcriptome, metabolome, and BRD phenotype of feedlot crossbred cattle. The findings may be useful for the development of genomic selection strategies for BRD susceptibility, and for the development of new diagnostic and therapeutic tools.

2023-01-11 | GSE217317 | GEO

Multipopulational transcriptome analysis of beef cattle at arrival to identify and further validate candidate biomarkers for predicting clinical bovine respiratory disease

Project description:Objective: Classify and further validate BRD-associated genes and mechanisms found to be differentially expressed at facility arrival in post-weaned beef cattle from multiple independent populations. Our approach was to profile and compare at-arrival whole blood transcriptomes of post-weaned beef cattle that failed to develop symptoms associated with clinical BRD and cattle that ultimately were diagnosed and treated for clinical BRD within the first 28 days of facility arrival, further stratifying diseased animals into severity cohorts. Methods: Whole blood RNA profiles were generated via NGS approach, using an Illumina Novaseq 6000. Individual samples were processed using an adaptation of the Revised Tuxedo Suite protocol (Tuxedo 2), then analyzed in R using edgeR with likelihood-ratio testing. Genes were considered differentially expressed with an FDR cutoff of 0.05. Downstream analysis involved PCA, gene ontology term enrichment, pathway analysis, protein-protein interaction networking, and IPA. Results: 132 differentially expressed genes were identified between all three cohorts. Biological processes and pathways primarily involved neutrophilic activity, antimicrobial killing, cell cornification, and inflammatory signaling/regulation. Conlusions: This study corroborated genes and pathways identified in our previous work, while providing novel genes and pathways for future analysis.

2021-12-13 | GSE161396 | GEO

The nasopharyngeal microbiota of cattle with bovine respiratory disease

Project description:The nasopharyngeal microbiota of healthy cattle vs. cattle diagnosed with BRD in a commercial feedlot setting was compared using a high-density 16S rRNA microarray (Phylochip). Nasopharyngeal samples were taken from both groups of animals (n=5) at feedlot entry (day 0) and >60 days later.

2015-06-21 | E-MTAB-3430 | biostudies-arrayexpress

nCounter NanoString Human Immunology V2 panel

Project description:mRNA expression in human bronchial epithelial cells unstimulated was compared with mRNA expression of cells stimulated with poly (I:C) (viral mimic) and poly (I:C) in combination with imiquimod (TLR7 agonist; Treatment). The aim was to assess the effect of imiquimod treatment on poly (I:C)-dependent changes in bronchial epithelium from asthmatics.

2021-08-02 | GSE181281 | GEO

Nanostring ncounter NHP Immunology V2 panel

Project description:The gene expression analysis of the RNA samples were analyzed with NanoString Non Human Primate immunology panel comprised of 754 immune related gene

2021-12-25 | GSE192584 | GEO

Effect of vaccination, marketing strategy, and bovine respiratory disease development on at arrival transcriptomes of recently weaned beef cattle

Project description:Jugular whole blood samples (Tempus Blood RNA tubes) were collected and utilized from 43 cattle, specifically from all cattle diagnosed with bovine respiratory disease (BRD) during Texas backgrounding (n=32) and randomly selected cattle never treated for BRD (n=11). Total RNA was isolated from blood samples, and were library prepared and sequenced via Illumina NovaSeq 6000 S4 chemistry. Samples were bioinformatically processed in a HISAT2/StringTie2 pipeline, and stratified based on BRD diagnosis and marketing strategy (Auction versus Direct marketing and transport). Differential expression analysis was conducted in R, utilizing an additive, multifactor generalized linear model and blocking for Mississippi pasture and vaccination status. Genes were considered differentially expressed with an FDR cutoff of 0.05. The objective of this study was to identify differentially expressed genes and mechanisms within and across marketing cohorts with and without BRD.

2023-03-09 | GSE218061 | GEO

MicroRNA expression in bovine satellite cells at 6th day of in vitro differentiation - myotubes (Hereford & Limousin vs Holstein-Freisian cattle)

Project description:miRNA profiling of bovine satellite cells (BSC) differentiated into myotubes (6th day of in vitro differentiation). BSC isolated from m. semitendinosus of beef (Hereford & Limousine) and dairy (Holstein-Friesian) cattle. Goal was to determine differences in miRNA expresion during in vitro myogenesis in beef vs dairy cattle used as a control.

2018-01-27 | GSE73778 | GEO