Transcriptomics

Dataset Information

22

Discovery of novel imprinted genes by transcriptional analysis of parthenogenetic embryonic stem cells


ABSTRACT: Parthenogenetic embryonic stem cells (PESCs) may have future utility in cell replacement therapies. We examined genome-wide mRNA expression profiles of monkey PESCs relative to ESCs derived from fertilized embryos. Several known paternally-imprinted genes were in the highly down-regulated group in PESCs compared to ESCs. Allele specific expression analysis of paternally-imprinted genes, i.e., those genes whose expression is down-regulated in PESCs, led to the identification of one novel candidate that was exclusively expressed from a paternal allele. Our findings suggest that PESCs could be used as a model for studying genomic imprinting and in the discovery of novel imprinted genes. Keywords: gene expression Overall design: The transcriptomes of rhesus monkey embryonic stem cell lines derived from IVF-produced embryos (Oregon Rhesus Macaque Embryonic Stem, ORMES-22) were compared with rhesus monkey parthenogenetic embryonic stem cell lines (heterozygous rhesus Parthenogenetic embryonic stem cell lines, rPESC-2) and homozygous rhesus Parthenogenetic embryonic stem cell lines, ORMES-9). Moreover, the transcriptomes of rPESC-2 line were also compared with ORMES-9. Finally, the adult somatic skin fibroblasts were analyzed. Three biological replicates of each cell line (A, B, C) were analyzed.

INSTRUMENT(S): [Rhesus] Affymetrix Rhesus Macaque Genome Array

ORGANISM(S): Macaca mulatta  

SUBMITTER: Hathaitip Sritanaudomchai   

PROVIDER: GSE17964 | GEO | 2010-06-21

SECONDARY ACCESSION(S): PRJNA119933

REPOSITORIES: GEO

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-05-02 | E-GEOD-17964 | ArrayExpress
2006-08-17 | GSE4446 | GEO
2007-08-29 | GSE7748 | GEO
2011-05-31 | GSE27362 | GEO
| GSE75817 | GEO
2011-06-10 | E-GEOD-27362 | ArrayExpress
2009-09-10 | GSE17981 | GEO
2014-05-02 | E-GEOD-17981 | ArrayExpress
2014-10-19 | E-GEOD-61420 | ArrayExpress
| PRJNA143329 | ENA