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Mammalian UPF3A and UPF3B activate NMD independently of their EJC binding

ABSTRACT: Nonsense-mediated mRNA decay (NMD) can occur independently of some NMD factors such as UPF3B. We have performed total RNA-Seq in HCT116 cells under NMD factor knockout (with CRISPR-Cas9) and/or knockdown (with siRNA transfection) conditions to identify mRNA substrates regulated by different NMD factors. We have also performed RIPiT-Seq to identify the footprints of three compositionally distinct EJCs that contain the following pairs of proteins: MAGOH-EIF4A3, UPF3B-EIF4A3 or CASC3-EIF4A3 in WT HCT116 cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE179843 | GEO | 2022/04/23

REPOSITORIES: GEO

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Mammalian UPF3A and UPF3B activate NMD independently of their EJC binding

Project description:Mammalian UPF3A and UPF3B activate NMD independently of their EJC binding

| PRJNA745322 | ENA

Genome-wide consequences of compromised NMD and their relavence for variable clinical phenotype of patients with UPF3B mutations [CNV]

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