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Single-cell RNA-seq of lung cells following bacterial pneumonia with or without LIF blockade

ABSTRACT: C57BL/6 mice were intratracheally challenged for 24 hours with saline or E. coli (1 x 10^6 CFU) with or without LIF neutralization. Left lobes were collected and digested into single-cell suspensions for FACS. Equal ratios of CD45+ (leukocytes), CD45-EpCAM+ (epithelium), and CD45-EpCAM- (other) cells were pooled from a mouse undergoing each condition.

ORGANISM(S): Mus musculus

PROVIDER: GSE179855 | GEO | 2022/02/17

REPOSITORIES: GEO

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Expression data from mouse lung epithelial cells during E. coli pneumonia in the presence or absence of LIF neutralization (6 h)

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