Influence of IWS1 TND-interacting motifs (TIMs) on gene expression patterns [RNA-seq]

ABSTRACT: To reveal the influence of TND-interacting motifs (TIMs), which bind TFIIS N-terminal domains (TNDs), we prepared KELLY cells expressing either wild-type IWS1-FLAG or IWS1-FLAG containing disruptive mutations in the unstructured TIM3 region. Following lentiviral transduction of either WT or M3 IWS1-FLAG, we prepared mRNA from these samples and performed expression profiling using RNA-seq. Analysis of differential gene expression provided markers that are differentially regulated by TIM3 of IWS1.

ORGANISM(S): Homo sapiens

PROVIDER: GSE179911 | GEO | 2021/11/26

REPOSITORIES: GEO