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Influence of IWS1 TND-interacting motifs (TIMs) on transcription elongation [PRO-seq]

ABSTRACT: To reveal the influence of TND-interacting motifs (TIMs), which bind TFIIS N-terminal domains (TNDs), we prepared KELLY cells expressing either wild-type IWS1-FLAG or IWS1-FLAG containing disruptive mutations in the unstructured TIM3 region. Following lentiviral transduction of either WT or M3 IWS1-FLAG, we prepared PRO-seq libraries from these samples and examined elongation profiles. Analysis of elongation profiles revealed that TIM3 disruption induces pervasive defects of transcription elongation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE179916 | GEO | 2021/11/26

REPOSITORIES: GEO

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