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Transcriptome analysis of 16 DAP seeds of Illinois protein lines.

ABSTRACT: Illinois protein lines (IPL) comprise lines known as Illinois high protein (IHP), Illinois low protein (ILP), Illinois reverse-low protein (IRLP) and Illinois reverse-high protein (IRHP) lines. These lines represent over a century old experiment containing lines that represent various levels of nitrogen metabolism potential as a function of their grain protein concentration. Microarray experiment was conducted using 16 DAP seeds from the above mentioned four IPLs. Keywords: Genotype response

ORGANISM(S): Zea mays

PROVIDER: GSE18006 | GEO | 2010/01/14

SECONDARY ACCESSION(S): PRJNA119907

REPOSITORIES: GEO

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Transcriptome analysis of 16 DAP seeds of Illinois protein lines.

Project description:Illinois protein lines (IPL) comprise lines known as Illinois high protein (IHP), Illinois low protein (ILP), Illinois reverse-low protein (IRLP) and Illinois reverse-high protein (IRHP) lines. These lines represent over a century old experiment containing lines that represent various levels of nitrogen metabolism potential as a function of their grain protein concentration. Microarray experiment was conducted using 16 DAP seeds from the above mentioned four IPLs. Keywords: Genotype response Technical replicates, dye swaps, technical replicates of dye swaps, no biological replicates

2010-01-13 | E-GEOD-18006 | biostudies-arrayexpress

Genotype changes in 8DAP leaf

Project description:Genotype dependent gene expression changes in the leaves of Illinois protein lines at 8 days after pollination (DAP). Illinois protein lines (IPL) comprise lines known as Illinois high protein (IHP), Illinois low protein (ILP), Illinois reverse-low protein (IRLP) and Illinois reverse-high protein (IRHP) lines. These lines represent over a century old experiment containing lines that represent various levels of nitrogen metabolism potential as a function of their grain protein concentration. Microarray experiment was conducted using 8 DAP leaves from the above mentioned four IPLs. Keywords: Genotype response

2009-12-11 | GSE17971 | GEO

Genotype changes in 8DAP leaf

2010-05-18 | E-GEOD-17971 | biostudies-arrayexpress

Transcriptomic analysis of maize developing earshoot, IBMRIL X IHP1 hybrid

Project description:Maize earshoot is a metabolic sink espcially related to nitrogen metabolism. Studies on the transcriptomic and metabolic changes occuring in earshoot can provide interesting answers about the nitrogen metabolic potential of the maize variety under study. Intermated B73 X Mo17 recombinant inbred lines (IBMRIL) belong to a predominantly used mapping population. Illinois high protein (IHP) line is a metabolic extreme with respect to nitrogen mebolism with around 30% seed protein. Developing earshoots from this genotype grown at nitrogen-deficient and nitrogen-sufficient conditions were sampled, processed and analyzed through microarray technology. IBMRIL population was crossed to IHP to find the allelic interaction related to nitrogen metabolism. Microarray experiment was conducted using the developing earshoots from IBMRIL X IHP1 lines that deffered in their grain yield significantly. Keywords: Yield response

2010-02-04 | GSE17953 | GEO

Transcriptomic analysis of maize developing earshoot, IBMRIL X IHP1 hybrid

2010-02-03 | E-GEOD-17953 | biostudies-arrayexpress

Transcription profiling of mouse embryonic fibroblasts from XIAP -/- animals after TNFa stimulation

Project description:XIAP (X chromosome-linked inhibitor of apoptosis; ILP, BIRC4) is involved not only in apoptosis, but also in signal tranduction in the NF-kappaB, TGF-beta, bone morphogenic protein, and JNK signalling pathways. We have analyzed whether XIAP deletion leads to changes in gene expression, particularly after inflammatory stimulation. Experiment Overall Design: Fibroblasts from wild-type and XIAP knockout mice were analyzed with and without TNFa stimulation (4 hours) using Affymetrix U74Av2 arrays.

2008-11-15 | E-GEOD-3700 | biostudies-arrayexpress

In vivo proximity labeling and sequencing (IPL-seq) for SNRPN70 in 293T-Rex

Project description:This proof-of-principle experiment was designed to demonstrate the feasibility of proximity labeling for RNAM-bM-^@M-^Sprotein interactions IPL-seq on 293T-Rex expressing MSA-SNRPN70 (sample) or NFH-SNRPN70 (control)

2014-10-24 | E-GEOD-55370 | biostudies-arrayexpress

ROR1-targeted delivery of miR-29b results in epigenetic reprogramming with p21-dependent cell cycle arrest

Project description:RNAseq analysis of purified splenic CD19+ B lymphocytes from the hRORxTCL1 mouse model treated with 2A2-miR-29b-ILP or 2A2-scramble-ILP. Moreover, 128 of the 233 differentially expressed genes are related to cell growth and proliferation.

2021-09-01 | GSE120461 | GEO

Project description:Reverse phase protein arrays (RPPAs)

| PRJNA289514 | ENA

Generation of induced progenitor-like cells from mature epithelial cells using interrupted reprogramming

Project description:A suitable source of progenitor cells is required to attenuate disease or affect cure. We present a novel interrupted reprogramming strategy to generate induced Progenitor-Like (iPL) cells using carefully timed -expression of induced Pluripotent Stem cell reprogramming factors (Oct4, Sox2, Klf4 and c-Myc; OSKM) from non-proliferative Club cells. Interrupted reprogramming allowed controlled expansion yet preservation of lineage commitment. Under clonogenic conditions, iPL cells expanded and functioned as a bronchiolar progenitor-like population to generate mature Club cells, mucin-producing goblet cells, and CFTR-expressing ciliated epithelium. In vivo, iPL cells can repopulate CFTR-deficient epithelium. This interrupted reprogramming process could be metronomically applied, to achieve controlled progenitor-like proliferation. By carefully controlling the duration of -expression of OSKM, iPL cells don't become pluripotent and maintain their memory of origin and retain their ability to efficiently return to their original phenotype. A generic technique to produce highly specified populations may have significant implications for regenerative medicine. Microarray analysis of genome-wide transcriptional changes showed Club-iPL cells have minimal expression of ES cell-related genes (e.g. Nanog, Utf1, Dapp4) and maintained significant overlapping expression of Club cell-related genes. Further analysis showed that the expression of large numbers of genes temporally changed during Dox-driven expansion of Club cells but returned upon withdrawal of the factors.

2017-10-24 | GSE105775 | GEO

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