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On/Off targets INDEL analysis by Next Generation Sequencing

ABSTRACT: SpCas9 INDEL frequency was evaluated at both the on- and off-target sites of guideRNA targeting either the murine Rhodopsin or Albumin by Next Generation Sequencing. Minimal INDELs were found at the off target sites evaluted, while SpCas9 activity was predominantly on-target.

ORGANISM(S): Mus musculus

PROVIDER: GSE180117 | GEO | 2021/11/11

REPOSITORIES: GEO

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