Genomics

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Defining the nature of human pluripotent stem cell-derived interneurons via single cell analysis


ABSTRACT: We applied single nuclei and single cell transcriptomics methods to model interneuron development with interneurons derived from human PSCs. We find that PSC-derived interneurons capture stages of development prior to mid gestation, but only represent a minority of potential subtypes that are found in the brain. Comparison of these interneurons to those found in the adult brain highlighted decreased expression of synapse-related genes, reflective of their electrophysiological immaturity. Together, these analyses provide a full characterization of PSC-derived interneurons.

ORGANISM(S): Homo sapiens

PROVIDER: GSE180132 | GEO | 2021/07/15

REPOSITORIES: GEO

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