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Dataset Information

25

Comparison of murine colonic mucosal DNA from postnatal day 90 (P90) to postnatal day 30 (P30) by MSAM


ABSTRACT: DNA methylation profiling of colonic mucosal DNA between P90 and P30 mice. 0.5ug of DNA was serially digested with SmaI and XmaI followed by an adaptor ligation and adaptor mediated PCR amplification Overall design: Two independent P90 to P30 comparisons were performed as follows. Samples were labelled with Cy3 (P30) and Cy5 (P90) and two independent P90 to P30 comparisons were done on a 2x105k methylation specific amplification microarray (MSAM) containing 90,535 probes, covering 77% of the 31,019 SmaI intervals between 200 bp and 2 kb in the mouse genome (average 3.8 probes per interval)

INSTRUMENT(S): Agilent Mouse array 105k

ORGANISM(S): Mus musculus  

SUBMITTER: Richard Kellermayer 

PROVIDER: GSE18031 | GEO | 2009-09-30

SECONDARY ACCESSION(S): PRJNA119269

REPOSITORIES: GEO

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