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Studies on genomic imprinting in preimplantation embryos of non-human primates [WGBS]

ABSTRACT: Genomic imprinting resulted by asymmetric epigenome of parents is a conserved phenomenon restricting biased expression of genes on two alleles. Disorder of imprinting can cause severe defects and diseases. In mouse and human, most of imprinted genes are expressed in species-specific and tissue-specific style. Here, rhesus monkeys as non-human primates are used to investigate primate genomic imprinting. We got a novel candidate canonical imprinted genes list in genome-wide by integrated analysis of allele-specific DNase I hypersensitive sites, differential expressed genes and differential DNA methylation. Further analysis revealed that both DNA methylation and H3K27me3 have an effect on the expression of imprinted genes. Consistent with human, analysis of H3K27me3 showed that H3K27me3 undergoes reprogramming in pre-implantation. Thus, our data provides a genome-wide view of genomic imprinting in non-human primates and sheds light on the further understanding of epigenetic programming process of monkey.

ORGANISM(S): Macaca mulatta

PROVIDER: GSE180571 | GEO | 2025/12/22

REPOSITORIES: GEO

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Studies on genomic imprinting in preimplantation embryos of non-human primates [RNA-seq]

Project description:Genomic imprinting resulted by asymmetric epigenome of parents is a conserved phenomenon restricting biased expression of genes on two alleles. Disorder of imprinting can cause severe defects and diseases. In mouse and human, most of imprinted genes are expressed in species-specific and tissue-specific style. Here, rhesus monkeys as non-human primates are used to investigate primate genomic imprinting. We got a novel candidate canonical imprinted genes list in genome-wide by integrated analysis of allele-specific DNase I hypersensitive sites, differential expressed genes and differential DNA methylation. Further analysis revealed that both DNA methylation and H3K27me3 have an effect on the expression of imprinted genes. Consistent with human, analysis of H3K27me3 showed that H3K27me3 undergoes reprogramming in pre-implantation. Thus, our data provides a genome-wide view of genomic imprinting in non-human primates and sheds light on the further understanding of epigenetic programming process of monkey.

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