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Dihydrotestosterone induced changes in gene expression in primary and immortalized human corneal epithelial cells

ABSTRACT: Genome-wide analysis of dihydrotestosterone (DHT) induced changes in gene expression in primary and immortalized human corneal epithelial cells. Analysis of regulation of primary and immortalized human corneal epithelial cells by dihydrotestosterone at gene expression level. The hypothesis tested in the present study was that the androgen-eye interaction in ocular surface epithelial cells like corneal cells is influenced by androgens through regulation of the expression of multiple genes. Results provide important information of the differential regulation and comparitive analysis of numerous genes in response to dihydrotestosterone incubation in primary and immortalized human corneal epithelial cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE18093 | GEO | 2011/03/31

SECONDARY ACCESSION(S): PRJNA119381

REPOSITORIES: GEO

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Dihydrotestosterone induced changes in gene expression in primary and immortalized human corneal epithelial cells

Project description:Genome-wide analysis of dihydrotestosterone (DHT) induced changes in gene expression in primary and immortalized human corneal epithelial cells. Analysis of regulation of primary and immortalized human corneal epithelial cells by dihydrotestosterone at gene expression level. The hypothesis tested in the present study was that the androgen-eye interaction in ocular surface epithelial cells like corneal cells is influenced by androgens through regulation of the expression of multiple genes. Results provide important information of the differential regulation and comparitive analysis of numerous genes in response to dihydrotestosterone incubation in primary and immortalized human corneal epithelial cells. Total RNA was obtained from primary and immortalized human corneal epithelial cells treated for 5 days with 10 nM dihydrotestosterone (n=3) or vehicle (n=3). The RNA was then used with Illumina HumanHT-12 v3 Expression BeadChips to determine the effect of DHT on gene expression in the primary human corneal cells grown in our laboratory and the immortalized human corneal epithelial cell line developed in Dr. Rheinwald's laboratory [Rheinwald et al. MCB, 22 (14): 5157. (2002)] and charecterized in Dr. Ilene Gibson's laboratory [Gipson et al. IOVS, 44 (6): 2496. (2003)].

2011-03-31 | E-GEOD-18093 | biostudies-arrayexpress

Dihydrotestosterone induced changes in gene expression in immortalized human conjunctival epithelial cells

2011-03-31 | GSE18094 | GEO

Dihydrotestosterone induced changes in gene expression in immortalized human meibomian gland epithelial cells

2011-03-31 | GSE18091 | GEO

Dihydrotestosterone induced changes in gene expression in immortalized human meibomian gland epithelial cells

Project description:Genome-wide analysis of dihydrotestosterone (DHT) induced changes in gene expression in immortalized human meibomian gland epithelial cells. Analysis of regulation of immortalized human meibomian gland epithelial cells by dihydrotestosterone at gene expression level. The hypothesis tested in the present study was that the androgen-eye interaction in ocular surface epithelial cells like meibomian gland cells is influenced by androgens through regulation of the expression of multiple genes. Results provide important information of the differential regulation of numerous genes in response to dihydrotestosterone incubation of immortalized human meibomian gland epithelial cells. Total RNA was obtained from immortalized human meibomian gland epithelial cells treated for 72 hours with 10 nM dihydrotestosterone (n=3) or vehicle (n=3). The RNA was then used with Illumina HumanHT-12 v3 Expression BeadChips to determine the effect of DHT on gene expression in an immortalized human meibomian epithelial cell line developed in our laboratory.

2011-03-31 | E-GEOD-18091 | biostudies-arrayexpress

Dihydrotestosterone induced changes in gene expression in immortalized human conjunctival epithelial cells

Project description:Genome-wide analysis of dihydrotestosterone (DHT) induced changes in gene expression in immortalized human conjunctival epithelial cells. Analysis of regulation of immortalized human conjunctival epithelial cells by dihydrotestosterone at gene expression level. The hypothesis tested in the present study was that the androgen-eye interaction in ocular surface epithelial cells like conjunctival cells is influenced by androgens through regulation of the expression of multiple genes. Results provide important information of the differential regulation of numerous genes in response to dihydrotestosterone incubation of immortalized human conjunctival epithelial cells. Total RNA was obtained from immortalized human conjunctival epithelial cells treated for 96 hours with 10 nM dihydrotestosterone (n=3) or vehicle (n=3). The RNA was then used with Illumina HumanHT-12 v3 Expression BeadChips to determine the effect of DHT on gene expression in an immortalized human conjunctival epithelial cell line developed in Dr. Rheinwald's laboratory [Rheinwald et al. MCB, 22 (14): 5157. (2002)] and charecterized in Dr. Ilene Gibson's laboratory [Gipson et al. IOVS, 44 (6): 2496. (2003)].

2011-03-31 | E-GEOD-18094 | biostudies-arrayexpress

Effect of 5a-dihydrotestosterone on breast cancer cell line MDA-MB-453

2011-07-11 | GSE28305 | GEO

Homo sapiens

Project description:Dihydrotestosterone induced changes in gene expression in primary and immortalized human corneal epithelial cells

| PRJNA119381 | ENA

Effect of 5a-dihydrotestosterone on breast cancer cell line MDA-MB-453

Project description:Analysis of MDA-MB-453 breast cancer cells treated with the androgen 5a-dihydrotestosterone (DHT) for 6h, 16h and 48h to define the genes that are differentially regulated in response to DHT. MDA-MB-453 breast cancer cells were treated with 5a-dihydrotestosterone (DHT) for time course, followed by RNA extraction and hybridization on Affymetrix microarrays, in order to obtain the gene expression profiles at three time points. The vehicle treated samples are used as control.

2011-07-11 | E-GEOD-28305 | biostudies-arrayexpress

The effects of testosterone and lipopolysaccharide on gene expression in immortalized ocular surface and adnexal cells

Project description:Immortalized human corneal, conjunctival, and meibomian gland epithelial cells were pre-treated with dihydrotesteosterone (DHT) or vehicle, then exposed to lipopolysaccharide (LPS) and ligand binding protein (LBP) or vehicle, lysed, and subjected to microarray analysis of RNA expression.

2018-03-07 | GSE111496 | GEO

Adipose tissue of gonadectomized mice injected with dihydrotestosterone

Project description:Comparison of retroperitoneal adipose tissue of intact male mice and gonadectomized C57BL6 male mice. Gonadectomized mice were untreated or injected with dihydrotestosterone (DHT) and sacrificed 3 or 24 hours after injection. Keywords: other

2004-04-24 | GSE1091 | GEO

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