Differential transcriptome profiling of P. aeruginosa PAO1 with oxythiamine treatment

ABSTRACT: We report antibacterial potency of oxythiamine, which is a known transketolase inhibitor in eukaryotes. Oxythiamine inhibited the growth of P. aeruginosa PAO1 and clinical isolates in thiamine-depleted medium only. Metabolic and trascriptomic analyses indicated that oxythiamine was converted to oxythiamine pyrophosphate that subsequently inhibited several vitamin B1-dependent enzymes. Furthermore, a system-level perturbation of thiamine metabolism sensitized P. aeruginosa to multiple small-molecules informing the potential development of a rational drug combination.

ORGANISM(S): Pseudomonas aeruginosa

PROVIDER: GSE181354 | GEO | 2021/08/03

REPOSITORIES: GEO