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Transcriptomic changes in FOXP3 deficient CD8+ T cells

ABSTRACT: Characterization of the functional differences observed by the absence of FOXP3 in CD8+ T lymphocytes. Highly purified OT-1 Foxp3Scurfy and OT-1 Foxp3wt T cells from the corresponding BM chimeric mice were isolated and left unstimulated or stimulated with SIINFEKL peptide for 6h in culture medium. RNA was then isolated and sequenced for the analysis of differential expression of mRNA transcripts

ORGANISM(S): Mus musculus

PROVIDER: GSE181510 | GEO | 2022/01/02

REPOSITORIES: GEO

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In vitro FOXP3 knockdown in human PBMCs

Project description:Human FOXP3 antisense oligonucleotides (ASOs) target effects in primary iTregs gene expression. Tregs were isolated from human PBMCs from 5 different donors, subjected to one of 4 different treatments: (1) Untreated, (2) Control ASO 792169, (3) FOXP3 ASO 910959, (4) FOXP3 ASO 910956 under either stimulation with aCD3/aCD28 coated beads or left unstimulated.

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Foxp3 positive and negative cell comparison

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2017-02-15 | PXD001789 | Pride

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