MNET-seq on COMPASS-degron cells

ABSTRACT: We measured the the dwell time of existed paused RNAPII at the promoter by mammalian native elongating transcript sequencing (mNET-seq). We found a median RNAPII half-life of 5.3 min at protein-coding genes in DMSO pre-treated control mESCs, whereas the RNAPII half-life at these genes significantly slowdown to 8.96 min when pre-treated with Auxin in the DPY30-mAID degron cell. The data suggested that loss H3K4me3 directly slows down the release of paused RNAPII throughout genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE181686 | GEO | 2023/01/20

REPOSITORIES: GEO