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RNA-seq of Lin-CD34+ cord-blood cells overexpressing PLAG1-S or firefly Luciferase [OE]

ABSTRACT: To characterize transcriptional changes associated with gain of the transcription factor PLAG1-S that could contribute to transient ex vivo expansion of human cord-blood (CB) derived HSC we performed RNA-seq of 2 pools of Lin-CD34+ CB cells 72 hours following lentiviral introduction of ectopic PLAG1-S or firefly Luciferase (control).

ORGANISM(S): Homo sapiens

PROVIDER: GSE181976 | GEO | 2022/12/01

REPOSITORIES: GEO

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CUT&RUN of PLAG1-S global chromatin occupancy in Lin-CD34+ cord-blood cells [Cut & Run]

Project description:To characterize genome-wide chromatin occupancy of the transcription factor PLAG1-S that could contribute to transient ex vivo expansion of human cord-blood (CB) derived HSC we performed CUT&RUN. 3 pools of Lin-CD34+ CB cells transduced to overexpress 3xFLAG-PLAG1-S were used for anti-FLAG or anti-IgG control enrichment of specific PLAG1-S DNA binding sites.

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RNA-seq of Lin-CD34+ cord-blood cells expressing shRNA against PLAG1 or control [KD]

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Plag1 in the mouse Epididymis

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Plag1 overexpression cooperates with Evi1 overexpression and Gata1s mutation in leading to M7 leukemia

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Plag1 in the mouse Epididymis

Project description:Plag1 in the mouse Epididymis

| PRJNA590199 | ENA