Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The diurnal demethylation of intronic CpG-rich islands is instrumental in expression of circadian clock-controlled genes [liver]

ABSTRACT: To identify the methylation status of DNA at two circadian time points (i.e. 8am and 8pm) in liver samples of wild type C57BL6/J mice. The methylated DNA was isolated, prified and immunoprecipated at two time points using 5-methyl cytosine antibody(MeDIP Assay).

ORGANISM(S): Mus musculus

PROVIDER: GSE182146 | GEO | 2023/03/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The diurnal demethylation of intronic CpG-rich islands is instrumental in expression of circadian clock-controlled genes [ileum]

Project description:To identify the methylation status of DNA at two circadian time points (i.e. 8am and 8pm) in ileum samples of wild type C57BL6/J mice. The methylated DNA was isolated, prified and immunoprecipated at two time points using 5-methyl cytosine antibody(MeDIP Assay).

2023-03-08 | GSE182145 | GEO

The diurnal demethylation of intronic CpG-rich islands is instrumental in expression of circadian clock-controlled genes.

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-08 | GSE182147 | GEO

The diurnal demethylation of intronic CpG-rich islands is instrumental in expression of circadian clock-controlled genes.

Project description:The diurnal demethylation of intronic CpG-rich islands is instrumental in expression of circadian clock-controlled genes.

| PRJNA754781 | ENA

The diurnal demethylation of intronic CpG-rich islands is instrumental in expression of circadian clock-controlled genes [liver]

Project description:The diurnal demethylation of intronic CpG-rich islands is instrumental in expression of circadian clock-controlled genes [liver]

| PRJNA754783 | ENA

The diurnal demethylation of intronic CpG-rich islands is instrumental in expression of circadian clock-controlled genes [ileum]

Project description:The diurnal demethylation of intronic CpG-rich islands is instrumental in expression of circadian clock-controlled genes [ileum]

| PRJNA754782 | ENA

The circadian demethylation of a unique intronic deoxymethylCpG-rich island boosts the transcription of its cognate circadian clock output gene.

Project description:We demonstrate that there is a tight functional relationship between two highly evolutionary conserved cell processes, i.e., the circadian clock (CC) and the circadian DNA demethylation-methylation of cognate deoxyCpG-rich islands. We have discovered that every circadian clock-controlled output gene (CCG), but not the core clock nor its immediate-output genes, contains a single cognate intronic deoxyCpG-rich island, the demethylation-methylation of which is controlled by the CC. During the transcriptional activation period, these intronic islands are demethylated and, upon dimerization of two YY1 protein binding sites located upstream to the transcriptional enhancer and downstream from the deoxyCpG-rich island, store activating components initially assembled on a cognate active enhancer (a RORE, a D-box or an E-box), in keeping with the generation of a transcriptionally active condensate that boosts the initiation of transcription of their cognate pre-mRNAs. We report how these single intronic deoxyCpG-rich islands are instrumental in such a circadian activation/repression transcriptional process.

| S-EPMC9974474 | biostudies-literature

Diurnal transcriptome rhythms in mice with genetically ablated clock function in the suprachiasmatic nucleus (SCN)

Project description:Male KO (Syt10-Cre/Cre x Bmal1-flox/-) and CON (Syt10-Cre/Cre x Bmal1-+/-) mice were entrained to 12h:12h light:dark conditions for two weeks and sacrificed at 4 different time points (CT1, 7, 13, 19) on the second day after release into constant darkness conditions. Total RNA was isolated from epididymal white adipose tissue biopsies and subjected to microarray hybridization.

2016-09-21 | GSE81532 | GEO

Diurnal Gene Variation in Mouse Eye

Project description:The purpose of this study was to explore diurnal gene expression changes that occur in the RPE/Choroid/Sclera. Mice C57BL/6J were purchased from the Jackson Laboratory (Bar Harbor, ME) and raised to approximately 2 months of age and entrained to a 12hr/12hr light/dark cycle for two weeks. Eye cups from male mice were rapidly dissected and RPE/ choroid/sclera tissues were collected over three consecutive diurnal cycles at Zeitgeber time (ZT) 0.5, 1, 1.5, 4, 11, 13, 16, and 23 hrs, for a total of 24 time points. Three mice were used for each time point and dissections for dark time points were done under dim-red light. To generate a reference RNA for microarray hybridization, whole eyes from equal numbers of male and female mice were collected at ZT6 and ZT7. Time: Samples were collected at the indicated Zeitgeber time points

2015-08-09 | GSE40023 | GEO

Analysis of gene expression in grape berries due to diurnal effects (DIU_06).

Project description:This is a 24 hour time course experiment to investigate changes in gene expression in deseeded berries sampled every three hours within a 24 hour period at 2 time points during development i.e. T4 (preveraison) & T15 (postveraison). Changes in gene expression within the 24 hour period were analyzed to identify expression profiles that coincided with diurnal changes. The gene expression profiles were then to be compared between the two developmental time points T4 v T15). Keywords: Diurnal time course

2018-11-22 | GSE7751 | GEO

Analysis of gene expression in grape berries due to diurnal effects (DIU_05).

Project description:This is a 24 hour time course experiment to investigate changes in gene expression in deseeded berries sampled every three hours within a 24 hour period at 2 time points during development i.e. T5 (preveraison) & T17 (postveraison). Changes in gene expression within the 24 hour period were analyzed to identify expression profiles that coincided with diurnal changes. The gene expression profiles were then to be compared between the two developmental time points T5 v T17). Keywords: Diurnal time course

2018-11-22 | GSE7749 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data