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Genome-wide differential binding pattern of NFKB2/p52 under prolonged culture conditions

ABSTRACT: We report the application of ChIP-Seq analysis for high-throughput profiling of NFKB2/p52 binding pattern under prolonged culture conditions. Briefly, we investigate the genome wide binding behavior of endogenous p52 in WI-38 cells. The cells was cultured with regular DMEM medium for prolonged time (7 days). Chromatin immunoprecipitated DNA was converted to libraries for sequencing with HiSeq 4000. We find that there is a distinct binding pattern of p52 under prolonged culture condtion. This study provides a preliminary but solid basis to our understanding of NFKB2/p52 binding dynamics during aging process.

ORGANISM(S): Homo sapiens

PROVIDER: GSE182248 | GEO | 2022/04/13

REPOSITORIES: GEO

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Genome-wide differential binding pattern of NFKB2/p52 under prolonged culture conditions

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