Expression data from early anther
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
PROVIDER: GSE18225 | GEO | 2009/09/23
SECONDARY ACCESSION(S): PRJNA119611
REPOSITORIES: GEO
Similar Datasets
Project description:We performed microarray experiments with RNAs from dissected immature anthers (stages 4-7) from wild-type, dyt1 and ams mutant plants, using the Affymetrix ATH1 chip. Experiment Overall Design: Anthers from Arabidopsis thaliana were selected at 4-7 stages of early development for RNA extraction and hybridization on Affymetrix microarrays. Samples from two mutants and wild-type anther.
2009-09-25 | E-GEOD-18225 | biostudies-arrayexpress
Project description:In flowering plants, anther dehiscence and pollen release are essential for sexual reproduction. Anthers dehisce after cell wall degradation weakens stomium cell junctions in each anther locule, and desiccation creates mechanical forces that open the locules. Either effect or both together may break stomium cell junctions. The microRNA miR167 negatively regulates ARF6 and ARF8, which encode Auxin Response transcription Factors. Arabidopsis mARF6 or mARF8 plants with mutated miR167 target sites have defective anther dehiscence and ovule development. Null mir167a mutations recapitulated mARF6 and mARF8 anther and ovule phenotypes, indicating that MIR167a is the main miR167 precursor gene that delimits ARF6 and ARF8 expression in these organs. Anthers of mir167a or mARF6/8 plants overexpressed genes encoding cell wall loosening functions associated with cell expansion, and grew too large starting at flower stage 11. Experimental desiccation enabled dehiscence of miR167-deficient anthers, indicating competence to dehisce. Conversely, high humidity conditions delayed anther dehiscence in wild-type flowers. These results support a model in which miR167-mediated anther growth arrest permits anther dehiscence. Without miR167 regulation, excess anther growth delays dehiscence by prolonging desiccation.
2019-07-15 | GSE122436 | GEO
Project description:Maize anthers ranging in size from 0.5mm to 3.0mm in increments of 0.25mm were dissected and their cells isolated using the FX-Cell protocol. scRNA-seq libraries were prepped using the CEL-Seq2 protocol and sequenced with 150bp PE reads with Illumina HiSeq or NovoSeq. The developmental trajectories of the somatic cell layers were investigated.
2023-01-01 | GSE219091 | GEO
Project description:Transcriptional profiling of Arabidopsis buds comparing dyt1, tdf1, ams, ms188 mutants with wild type (Col ecotype), respectively. Goal was to determine the differential expression genes between the buds of mutant and wild type.
2017-08-12 | GSE102528 | GEO
Project description:Transcriptomes from multiple pre-meiotic stages of wild type, mac1, and msca1 maize anthers were characterized by microarray hybridization. The goal was to characterize the developmental progression as the anther specifies five cell types and grows rapidly precedeing meiotic entry. The stages characterized were immature anther primordia (0.15 mm long in maize) containing just stem cells, through somatic and germinal cell fate specification (0.20 and 0.25 mm), mitotic proliferation (0.4 mm), and finally the birth of the middle layer and tapetum (0.7 mm). To obtain cell-type specific markers, at 0.7 mm we also compared whole anthers to collections of laser-microdissected anther cell types including the archesporial cells (pre-meiotic germinal cells), nutritive layers (middle layer and tapetum) and structural layers (endothecium and epidemis) of the anther lobe. keyword: anther development, maize, male-sterile Three loop designs covered the early stages (up to 0.7 mm) with two replicates for each comparison. The first loop had 0.2 mm long anthers and compared wild type versus mac1 mutant versus msca1 mutant in a three vertex loop design. The second loop had four vertices and compared 0.15 mm WT anther primordia, 0.25 mm WT anthers, 0.4 mm WT anthers and finally 0.4 mm mac1 mutant anthers. The third had 0.7 mm anthers in a three vertex loop with the nutritive layers (middle layer and tapetum) at one vertex, the germinal pre-meiotic cells at another vertex, and whole anthers at a third vertex. The whole anther samples were also, separately and outside of the loop, compared in four replicates to the structural layers (endothecium and epidermis).
2013-05-01 | E-GEOD-43982 | biostudies-arrayexpress
Project description:Transcriptomes from multiple pre-meiotic stages of wild type, mac1, and msca1 maize anthers were characterized by microarray hybridization. The goal was to characterize the developmental progression as the anther specifies five cell types and grows rapidly precedeing meiotic entry. The stages characterized were immature anther primordia (0.15 mm long in maize) containing just stem cells, through somatic and germinal cell fate specification (0.20 and 0.25 mm), mitotic proliferation (0.4 mm), and finally the birth of the middle layer and tapetum (0.7 mm). To obtain cell-type specific markers, at 0.7 mm we also compared whole anthers to collections of laser-microdissected anther cell types including the archesporial cells (pre-meiotic germinal cells), nutritive layers (middle layer and tapetum) and structural layers (endothecium and epidemis) of the anther lobe. keyword: anther development, maize, male-sterile
2013-05-01 | GSE43982 | GEO
Project description:A series of microarray experiments by using RNA sources from UDT1-1 mutant type- and wild type-anthers at stages from meiosis to young microspore. In conjuction, microarray experiments by using RNA sources from wild type_Palea/lemmas in the flowering stage and wild type anther at pollen mitosis were performed in a time course design and compared to the microarrays of UDT1-1 mutant type- and wild type-anthers. Keywords: time-course
2005-10-01 | GSE2619 | GEO
Project description:Illumina small RNA sequence data from maize anthers at 3 stages - 1mm, 1.5mm, and 2mm. Plants were field-grown and then anthers dissected and grouped according to length. RNA was extracted using TriReagent protocol. Each of the 3 sample types were sequenced separately.
2011-07-28 | E-GEOD-30986 | biostudies-arrayexpress
Project description:A series of microarray experiments by using RNA sources from UDT1-1 mutant type- and wild type-anthers at stages from meiosis to young microspore. In conjuction, microarray experiments by using RNA sources from wild type_Palea/lemmas in the flowering stage and wild type anther at pollen mitosis were performed in a time course design and compared to the microarrays of UDT1-1 mutant type- and wild type-anthers. Keywords: time-course
2010-06-09 | E-GEOD-2619 | biostudies-arrayexpress
Project description:MALE STERILITY 35 (MS35/ MYB26) is a R2R3-type MYB transcription factor (Steiner-Large et al., 2003). MS35 is suggested to be a key regulator in the process of lignified secondary thickening in the anther endothecium which provides mechanical force for anther dehiscence (Dawson et al., 1999). The ms35 mutant bears non-dehiscent anthers and thus exhibits a male sterile phenotype. This microarray experiment aims to identify genes controlled by MS35 and thus establish the precise role MS35 plays during anther development. We have demonstrated that MS35 is expressed in the anther during pollen mitotic divisions. Comparisons of gene expression will be carried out between anthers from the Ler.gl (wild-type control) and ms35.gl (mutant) to identify genes with altered expression due to the ms35 mutation. The use of the gl marker will enable a prompt identification of the ms35 mutant amongst the segregating population. The wild-type control line was chosen to be Ler.gl which ensures that the changes of gene expression are not due to the gl mutation. Plants were grown in growth chambers at 22h of daylight (167 umol.m2.sec-1) at 24 C and 2h of night at 24 C. Developmental stages of anthers were defined by bud position and DAPI staining. As soon as the plants showed three fertile or sterile siliques, anther and filaments were collected from both lines at Pollen Mitosis I (PM I), bicellular, and PM II stage, separately. RNA was extracted using RNeasy Mini Kit (Qiagen, USA) and digested with DNase to eliminate genomic DNA contamination. The identified genes will be further analysed using bioinformatics and genomic (knockout) resources that are available for Arabidopsis.
2009-12-10 | GSE19267 | GEO