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Genome-wide maps of histone modification H3K4me3 in Daudi cells.

ABSTRACT: We report the updated method for CUT&Tag technology for high-throughput profiling of histone modifications or transcription factors in mammalian cells. By using using hand-made concanavalin A conjugated beads, we greatly improved handling and suspendability of cell-bound magentic beads and increased sensitivity for detecting peaks compared with conventional concanavalin A coated magnetic beads. This study provides a framework for the application of epigenome studies using mammalian cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE182254 | GEO | 2021/08/20

REPOSITORIES: GEO

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Project description:Preparation of stress-free concanavalin A-conjugated Dynabeads magnetic beads for CUT&Tag

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SAGE analysis of human glomerular endothelial cell

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2010-12-01 | GSE25760 | GEO

Single-base resolution methylome of 5-methylcytosine in mammalian transcriptome

Project description:mRNAs of human and mouse cell lines were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement. mRNAs were fragmented to ~100 nt and treated by Sodium bisulfite solution (pH 5.1) containing Hydroquinone. Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA) and performed sequencing on HiSeq2000/3000 (Illumina) in pair-end mode, creating reads with a length of 101 bp. Sequencing chemistry v2 (Illumina) was used and samples were multiplexed in two samples per lane.

2018-10-23 | GSE74771 | GEO

Single-base resolution methylome of 5-Methylcytosine in human and mouse transcriptome

Project description:mRNAs of siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues were purified using oligo (dT)-conjugated magnetic beads followed by RiboMinus treatement. mRNAs were fragmented to ~200 nt and treated by Sodium bisulfite solution (pH 5.1) containing Hydroquinone. Bisulfite treated mRNAs were reverse transcribed to cDNA using ACT random hexamer primers. The cDNAs were subjected to libraries construction using KAPA Stranded mRNA-Seq Kit (KAPA) and performed sequencing on HiSeq2500 (Illumina) in pair-end mode, creating reads with a length of 125 bp. Sequencing chemistry v4 (Illumina) was used and every sample was sequenced at one lane.

2017-04-19 | GSE93749 | GEO

RNA m5C Methylation in breast cancer using MeRIP-Seq

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2023-07-12 | GSE212444 | GEO

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