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Genomic and transcriptomic analysis of Bovine P.multocida serogroup A strains reveals insights into virulence attenuation

ABSTRACT: Pasteurella multocida is one of the main pathogens of bovine respiratory disease (BRD), which has brought great losses to the cattle industry. The Pm3 strain was a natural isolates, its pathogenic stronger and sensitive to fluoroquinolones. A highly fluoroquinolone resistant strain Pm64(MIC=64 μg/mL) was formed after continuous induction with subinhibitory concentration (1/2MIC) of enrofloxacin，with the enhanced growth characteristics and large attenuation of pathogenicity in mice. In this study, we report the whole genome sequence and the transcription profile by RNA-Seq of strain Pm3/Pm64. The results showed that there was not too much difference between the two strains at the genome level, 24 and 8 genes could be recognized were added and lost in the gene islands(GIs) of Pm64, Those gene involved in DNA binding, trehalose metabolism, material transportation, capsule synthesis, prophage, amino acid metabolism and other functions. 558 up-regulated and 568 down-regulated genes were found in Pm3 campared with Pm64, from which 20 virulence factor-related DEGs were screened. Genes differentially transcribed were mostly associated with capsular polysaccharide(CPS), lipopolysaccharide (LPS), lipooligosaccharide (LOS), Iron utilization and biofilm composition. We speculated that the main mechanism of virulence attenuation after the formation of resistance of Pm64 comes from the change of the expression profile of these genes. This report helps to elucidate the toxicity targets of P. multocida serogroup A and provide helps for the following research on pathogenesis and antimicrobial drugs development.

ORGANISM(S): Bos taurus

PROVIDER: GSE182406 | GEO | 2021/08/21

REPOSITORIES: GEO

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