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Effects of BPA, BPAF, and BPS on Endochondral Ossification and on the Transcriptome of Ex Vivo Murine Limb Bud Cultures III

ABSTRACT: We report the results of treatment of embryonic limbs with bisphenol S. RNA sequencing was performed to measure differential expression as a result of exposure to BPS compared to DMSO treated control limbs.

ORGANISM(S): Mus musculus

PROVIDER: GSE182521 | GEO | 2023/07/31

REPOSITORIES: GEO

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Effects of BPA, BPAF, and BPS on Endochondral Ossification and on the Transcriptome of Ex Vivo Murine Limb Bud Cultures II

Project description:We report the results of treatment of embryonic limbs with bisphenol AF. RNA sequencing was performed to measure differential expression as a result of exposure to BPAF compared to DMSO treated control limbs.

2023-07-31 | GSE182046 | GEO

Effects of BPA, BPAF, and BPS on Endochondral Ossification and on the Transcriptome of Ex Vivo Murine Limb Bud Cultures I

Project description:We report the results of treatment of embryonic limbs with bisphenols A. RNA sequencing was performed to measure differential expression as a result of exposure compared to DMSO treated control limbs.

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Effects of BPA, BPAF, and BPS on Endochondral Ossification and on the Transcriptome of Ex Vivo Murine Limb Bud Cultures

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-07-31 | GSE182523 | GEO

Effects of an environmentally relevant mixture of organophosphate esters derived from house dust on endochondral ossification in murine limb bud cultures

Project description:Organophosphate esters (OPEs) are used widely as flame retardants and plasticizers but much remains unknown about their potential toxicity. Previously, we reported that four individual OPEs suppress endochondral ossification in murine limb bud cultures. However, real-life exposure is to complex OPE mixtures. In the present study, we tested the hypothesis that a Canadian household dust-based OPE mixture will affect endochondral ossification in gestation day 13 CD1 mouse embryo limb buds expressing fluorescent markers for the major cell populations involved in the process: COL2A1-ECFP (proliferative chondrocytes), COL10A1-mCherry (hypertrophic chondrocytes), and COL1A1-YFP (osteoblasts). Limbs were cultured for six days in the presence of vehicle or dilutions of the OPE mixture (1/1,000,000, 1/600,000, and 1/300,000). All three OPE mixture dilutions affected cartilage template development and the progression of endochondral ossification, as indicated by the fluorescent markers. The expression of Sox9, the master regulator of chondrogenesis, was unchanged, but the expression of Runx2 and Sp7, which drive chondrocyte hypertrophy and osteoblastogenesis, was dilution-dependently suppressed. RNA sequencing revealed that exposure to the 1/300,000 dilution of the OPE mixture for 24 h downregulated 153 transcripts and upregulated 48 others by at least 1.5-fold. Downregulated transcripts were enriched for those related to the immune system and bone formation. In contrast, upregulated transcripts were enriched for those with stress response functions known to be regulated by ATF4 activation. Thus, exposure to the mixture of OPEs commonly found in house dust may have adverse effects on bone formation.

2020-12-28 | GSE157940 | GEO

Effects of BPA, BPF and BPS treatments on mESC EB-based global and neural differentiations.

Project description:Purpose: the goal of this study is to determine the changes in gene expression during EB differentiation after BPA, BPF and BPS treatments, as indications of potential developmental toxicity. Methods: Transcriptomics profiling for EB samples treated with 100 nM BPA, BPF, BPS or DMSO control at different time points were generated by RNA-seq, using a BGISEQ-500 platform. Reads were filtered and aligned to the reference genome with Bowtie2. Results: BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, analogous gene categories were differentially regulated by the three chemicals, at each time point, particularly the ones involved in cell-matrix and cell-cell adhesions, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer.

2019-09-30 | GSE120224 | GEO

Effects of an Environmentally Relevant Mixture of Organophosphate Esters Derived From House Dust on Endochondral Ossification in Murine Limb Bud Cultures.

Project description: Not available

| S-EPMC7916738 | biostudies-literature

Effects of BPS and BPF on transcriptional networks of neuronal cells

Project description:Over the past several years, replacement chemicals for bisphenol A have been manufactured and used as additives in sportswear, plastic bottles, food packaging, and in other products. More recently, replacement products for BPA are under investigation for toxicity, but there are little data on the potential for neurotoxicity. To address this gap, differentiated human SH-SY5Y cells were treated with a range of concentrations of BPS and BPF to better understand mechanisms of toxicity.

2024-04-27 | GSE217951 | GEO

Peptidomics analysis of urine from IC/BPS patients and healthy controls

Project description:Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating pain disorder of the bladder and urinary tract with poorly understood etiology. A definitive diagnosis of IC/BPS can be challenging because many symptoms of IC/BPS are shared with other urological disorders. An analysis of urine presents an attractive and non-invasive resource for monitoring and diagnosing IC/BPS. Here, a non-targeted LC-MS and LC-MS/MS-based peptidomics analysis of urine samples collected from IC/BPS patients were compared to urine samples from asymptomatic controls.

2022-06-06 | PXD031843 | Pride

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2010-11-17 | E-GEOD-25394 | biostudies-arrayexpress

The impact of perinatal exposure to 2,4-BPS, BPSIP, BPS and E2 on the offspring of ICR mice

Project description:To investigate the effects of 2,4-BPS, BPSIP, BPS and E2 environmental hormones on offspring mice, we established a maternal exposure model from the first day of pregnancy until the end of lactation We subsequently fed the progeny mice a standard diet until 10 weeks of age, harvested their livers, and conducted sequencing analysis

2024-04-06 | GSE232892 | GEO

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