Dataset Information


Leukocyte accumulation in graft blood vessels during self-limiting acute rejection of rat kidneys Immunobiology

ABSTRACT: Acute rejection episodes trigger chronic renal allograft vasculopathy. Numerous leukocytes, predominantly monocytes, accumulate in graft blood vessels during reversible acute rejection preceding chronic rejection of rat kidneys. We speculate that they contribute to transplant vasculopathy and set out to characterize them. Allogeneic renal transplantation was performed in the Fischer 344 to Lewis rat strain combination, Lewis isografts served as controls. Leukocytes were harvested by intensive perfusion of graft blood vessels and subjected to flow cytometry, quantitative RT-PCR and genome-wide transcriptional profiling. Overall design: Kidneys of LEW and F344 rats were transplanted in LEW rats. Five biological replicates were performed for both isogenic and allogenic transplantation. Transcriptomes of allogenics were compared to isogenics on 5 dual-color hybridizations.

INSTRUMENT(S): Agilent-013162 Whole Rat Genome Microarray G4131A (Feature Number version)

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Jochen Wilhelm 

PROVIDER: GSE18266 | GEO | 2010-09-24



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