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Comparison of gene expression regulated by WRKY6

ABSTRACT: WRKY6-overexpressing line 35S:WRKY6-9, knockout mutant wrky6-1 and wild-type seedlings were used to analyze genes whose expression are controlled under WRKY6.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE18273 | GEO | 2009/12/07

SECONDARY ACCESSION(S): PRJNA117945

REPOSITORIES: GEO

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Comparison of gene expression regulated by WRKY6

Project description:WRKY6-overexpressing line 35S:WRKY6-9, knockout mutant wrky6-1 and wild-type seedlings were used to analyze genes whose expression are controlled under WRKY6. Seven-day-old seedlings of wild-type, 35S:WRKY6-9 and wrky6-1 mutant were harvested for RNA extraction.

2009-12-07 | E-GEOD-18273 | biostudies-arrayexpress

Analysis of genes regulated by an Arabidopsis transcription factor, Dof3.2

Project description:To obtain information on which genes are regulated by an Arabidopsis transcription factor Dof3.2, we treated Arabidopsis transgenic 35S::Dof3.2-GR seedlings with dexamethasone (DEX), then performed DNA microarray analyses. T3 homozygous Dof3.2-GR transgenic line was used.

2020-03-01 | GSE58064 | GEO

Transcript profiling of Arabidopsis thaliana transgenic seedlings constitutively overexpressing UGT74E2 (35S::UGT74E2).

Project description:Transcript profiling of transgenic Arabidopsis thaliana seedlings constitutively overexpressing UGT74E2 (35S::UGT74E2).

2009-09-19 | E-MEXP-2358 | biostudies-arrayexpress

Transcriptome analysis of Arabidopsis plants that overexpress green fluorescent protein (GFP) and GFP-LOV kelch protein 2 (GFP-LKP2)

Project description:LKP2 belongs to a group of blue light receptors in Arabidopsis thaliana. We performed DNA microarray analysis to identify genes whose expression levels are altered in LKP2-overexpressing plants. Agilent's Whole Arabidopsis Genome Oligo Microarray (G2519F, V4, 4x44K) was used. Comparison between 35S:GFP overexpressing- and 35S:GFP-LKP2 overexpressing- plants (Yasuhara et al. 2004) was performed.

2012-04-24 | E-GEOD-37197 | biostudies-arrayexpress

Analyses of genes regulated by the N-terminal photolyase homology region (CNT1/PHR) of CRY1 by RNA-seq

Project description:Total RNA was extracted from samples including 35S::CNT1-NLS-YFP;cry1 and 35S::CNT1-G283E-NLS-YFP;cry1 seedlings grown in continuous blue light and subjected to high throughput sequencing. Previous studies reported that the C-terminal domain of CRY1 regulates hypocotyl elongation and blue light-regulated genes. This study reveals that CNT1 overexpression also regulates blue light-regulated genes, supporting the role of CNT1 in hypocotyl elongation at transcriptomic level. mRNA from blue light-grown CNT1 or CNT1 variant (G283E) overexpressors was sequenced to analyze the genes regulated by CNT1.

2016-07-25 | E-GEOD-59763 | biostudies-arrayexpress

Identification of genes cooperatively regulated by cytokinin and HD-ZIP III transcription factors

Project description:In Arabidopsis thaliana, cytokinin responsive B-type ARR transcription factors and HD-ZIP III transcription factors such as REVOLUTA (REV), act cooperatively as master regulators of shoot regeneration. To identify the downstream targets of ARR-HD-ZIP III transcriptional complex, we used an inducible line of REV, 35S::FLAG-GR-rREV, in which FLAG-tagged miR165/6-non-targetable form of REV (rREV)-GR fusion protein was expressed from 35S promoter. DEX treatment induced activation of REV by translocation of FLAG-GR-rREV fusion protein from cytoplasm to the nucleus. We treated 35S::FLAG-GR-rREV seedlings with 6-benzylaminopurine (6-BA, a cytokinin), dexamethasone (DEX), or 6-BA+DEX for 2 hours. Total RNAs were extracted and subjected to Agilent Arabidopsis Gene Expression Microarray analyses. The differentially expressed genes (>1.5-fold, p<0.05) were identified.

2014-01-29 | GSE54469 | GEO

knockout-Transcriptomic analyses approach to identify genes associated with organ growth downstream TCTP in Arabidopsis

Project description:ra09-02_tctp - knockout - Identification of effector genes that act downstream of AtTCTP, using knockout ( tctp-2), RNAi expressing line (35S::RNAi-AtTCTP) and over expressing line (35S::AtTCTP) - transcriptome analysis in leaf of tctp-2 knock out versus wild type Keywords: gene knock out

2011-03-17 | GSE19847 | GEO

rnai-Transcriptomic analyses approach to identify genes associated with organ growth downstream TCTP in Arabidopsis

Project description:ra09-02_tctp - rnai - Identification of effector genes that act downstream of AtTCTP, using knockout ( tctp-2), RNAi expressing line (35S::RNAi-AtTCTP) and over expressing line (35S::AtTCTP) - transcriptome analysis in leaf of 35S::RNAi-AtTCTP versus wild type Keywords: normal vs rnai mutant comparison

2011-03-17 | GSE19849 | GEO

over expression-Transcriptomic analyses approach to identify genes associated with organ growth downstream TCT...

Project description:ra09-02_tctp - over expression - Identification of effector genes that act downstream of AtTCTP, using knockout ( tctp-2), RNAi expressing line (35S::RNAi-AtTCTP) and over expressing line (35S::AtTCTP) - transcriptome analysis in leaf of 35S::AtTCTP versus wild type Keywords: gene knock in (transgenic)

2011-03-17 | GSE19848 | GEO

Transcriptome analysis of genes regulated by overexpression of LATERAL ORGAN BOUNDARIES (LOB) in Arabidopsis thaliana

Project description:The Arabidopsis thaliana transcription factor LATERAL ORGAN BOUNDARIES (LOB) is expressed in the boundary between the shoot apical meristem and initiating lateral organs. To identify genes regulated by LOB activity, we used an inducible 35S:LOB-GR line. This analysis identified genes that are differentially expressed in response to ectopic LOB activity. 35S:LOB-GR and Col wild-type seedlings were treated with dexamethasone (DEX) or mock-treated. Three biological replicates were conducted for each treatment.

2011-12-07 | E-GEOD-34209 | biostudies-arrayexpress

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