Unknown

Dataset Information

20

IGH analysis in pro-B cells with H3K4me3, H3K4me2 and H3K9ac


ABSTRACT: STAT5 and IL-7 signaling are thought to control B-lymphopoiesis by regulating key transcription factor genes and activating VH gene segments at the Igh locus. Using conditional mutagenesis, we demonstrate that transgenic Bcl2 expression rescued the development of Stat5-deleted pro-B cells by compensating for the loss of Mcl-1. Ebf1 and Pax5 expression as well as VH recombination were normal in Bcl2-rescued pro-B cells lacking STAT5 or IL-7Ra. In agreement with this finding, STAT5-expressing pro-B cells contained little or no active chromatin at most VH genes. In contrast, Igk rearrangements were increased in STAT5- or IL-7Ra-deficient pro-B cells, consistent with direct binding of STAT5 to the intronic iEk enhancer in wild-type pro-B cells. Hence, STAT5 and IL-7 signaling control cell survival and suppress premature Igk recombination in early B-lymphopoiesis. Overall design: comparison of wt vs Rag2-/- pro-B cells

INSTRUMENT(S): GenAu MB BCELL v3.0

ORGANISM(S): Mus musculus  

SUBMITTER: Meinrad Busslinger   

PROVIDER: GSE18278 | GEO | 2010-07-28

SECONDARY ACCESSION(S): PRJNA117955

REPOSITORIES: GEO

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-05-01 | E-GEOD-18278 | ArrayExpress
2012-06-27 | E-GEOD-35914 | ArrayExpress
2012-06-27 | E-GEOD-35910 | ArrayExpress
| GSE63302 | GEO
| GSE81844 | GEO
2015-08-29 | E-GEOD-63302 | ArrayExpress
| GSE101606 | GEO
2012-11-12 | E-GEOD-40746 | ArrayExpress
2014-05-02 | E-GEOD-27214 | ArrayExpress
2014-05-02 | E-GEOD-27074 | ArrayExpress