Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sub-PEN-seq for identifying novel non-coding RNAs and their expression in cultured cells II

ABSTRACT: Small RNAs between 20-500nt were least explored. Here we used RNase H-based rRNA depletion and a serial of strategies to developed sub-PEN-seq method for identifying novel non-coding RNAs in Subcellular RNAs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE182843 | GEO | 2023/04/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

PEN-seq for identifying novel non-coding RNAs and their expression in cultured cells

Project description:Small RNAs between 20-500nt were least explored. Here we used RNase H-based rRNA depletion and a serial of strategies to developed PEN-seq method for identifying novel non-coding RNAs in total RNAs.

2023-04-10 | GSE160887 | GEO

RIP-PEN-seq for 15.5K in HEK293T cells

Project description:Here, we developed a RNA ImmunoPrecipitation coupled with sequencing of Paired-Ends of NcRNAs (RIP-PEN-seq) method to identify the full-length sequences of ncRNAs bound by 15.5K protein and discover a novel class of backward K-turn RNAs.

2023-04-10 | GSE160970 | GEO

RIP-PEN-seq for 15.5K in Hepa1-6 cells

2023-04-10 | GSE182757 | GEO

RIP-PEN-SHAPE-MaP for 15.5K-interacted RNAs

Project description:RNA secondary structure is crucial for RNA mentalism, including transcription, splicing, translation, RNA-binding protein interaction as well as turnover. The kink-turn (K-turn) structure motif is is an RNA three-dimensional (3D) structure that exists in all three primary phylogenetic domains and plays vital roles in RNA metabolism. In order to investigate the K-turn secondary structure in vivo, we combined SHAPE-Map and our RIP-PEN-seq to profile the RNA structure of 15.5K-interacted RNAs.

2023-04-10 | GSE220470 | GEO

Mycoplasma sp. Pen4 strain:Pen-4T

Project description:Mycoplasma sp. Pen4 strain:Pen-4T Genome sequencing

| PRJNA659024 | ENA

Comprehensive catalog of dendritically localized mRNA isoforms from sub-cellular sequencing of single mouse neurons

Project description:To compare the RNAs present in dendrites and somas of individual neurons, we manually separated the dendrites and soma of primary mouse hippocampal neurons using a micropipette and performed RNA-sequencing on each subcellular fraction such that we obtained the subcellular transcriptomes of the same cell.

2019-01-07 | GSE115480 | GEO

Lacticaseibacillus rhamnosus strain:Pen

Project description:Complete genome sequence of Lactobacillus rhamnosus Pen – a probiotic component of medicine preventing of antibiotic-associated diarrhoea in children.

| PRJNA359638 | ENA

Morphology and transcriptome of Atlantic salmon skin after seawater transfer, comparison of a semi-closed containment system and open net-pen

Project description:Development of semi-closed containment systems is a promising strategy to further expand Atlantic salmon production. The technology operates with higher production intensities where skin integrity is recognized as a central factor for animal health and welfare. This study investigate the skin development and its immune status in healthy Atlantic salmon growers reared in a semi-closed containment system and a traditional open net-pen system (control).

2018-05-05 | GSE114028 | GEO

The expression profiles of 82 novel and 245 known human imsRNAs

Project description:The expression profiling of 245 known and 82 novel imsRNAs (intermediate-size noncoding RNAs, 50-500nt, which were identified from human fetal brain tissue) across different human fetal tissues (brain, heart, liver, lung and spleen at the 24-week gestation stage), different fetal brain gestational stages (13w, 14w, 20w, and 24w) and two human brain tumor cell lines (SHSY-5Y and U251).

2010-12-21 | GSE26196 | GEO

Homo sapiens

Project description:Subcellular distribution of RNAs in HeLa cells

| PRJNA266343 | ENA