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FEAT overexpression in NIH3T3

ABSTRACT: To evaluate whether FEAT has cellular functions relevant in oncogenesis, FEAT was overexpressed in NIH3T3 cells, which only weakly express FEAT protein, and the alterations in genome-wide transcriptional profiles were analyzed by microarrays.

ORGANISM(S): Mus musculus

PROVIDER: GSE18299 | GEO | 2009/10/02

SECONDARY ACCESSION(S): PRJNA118027

REPOSITORIES: GEO

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Mus musculus

Project description:FEAT overexpression in NIH3T3

| PRJNA118027 | ENA

Role of SIRT7 overexpression in mouse fibroblast cells NIH3T3

Project description:Human SIRT7 GFP or GFP alone were overexpressed in NIH3T3 cells.In confluent conditions, SIRT7GFP overexpressing cells but not GFP expressing cells showed presence of distinct compact cellular colonies composed of small sized cells (ST7). Along with this SIRT7GFP overexpressing cells with long and fibroblast morphoogy(LT7) were also present. Single cell clones from these cells were expanded to form ST7 (Small SIRT7GFP expressing) and LT7 (Long SIRT7GFP expressing) lines. RNA from GFP expressing cells (SKGFP), ST7 and LT7 lines was then isolated and applied to microarray analysis to identify gene expression changes in two cell lines

2020-07-14 | GSE148486 | GEO

Transformation of NIH3T3 cells by the forced expression of wild-type YAP or its stabilized form

Project description:YAP is known as a proto-oncogene with which the aberrant expression causes numerous types of cancer. To elucidate the molecular mechanisms underlying the acquisition of cancerous traits of normal cells by YAP overexpression, the wild-type YAP or its stabilized form was overexpressed in NIH3T3 cells. We found that the forced expression of either wild-type YAP or its derivative alone was sufficient for transforming NIH3T3 cells and we then analyzed the global expression profiles of the control NIH3T3 cells or those overexpressing wild-type or stabilized YAP.

2019-08-21 | GSE103499 | GEO

Nutrient-dependent growth of NIH3T3 and NIH3T3 K-ras cell lines.

Project description:Expression profiling of normal NIH3T3 and transformed NIH3T3 K-ras cell lines grown for 72 hours in optimal glucose availability (25 mM glucose) or low glucose availability (1 mM). Low glucose induces apoptosis in transformed cells as compared to normal ones.

2011-07-14 | GSE29962 | GEO

Gene expression changes after Ctcfl overexpression in NIH3T3 cells.

Project description:The two paralogous zinc finger factors CTCF and CTCFL differ in expression such that CTCF is ubiquitously expressed, whereas CTCFL is found during spermatogenesis and in some cancer types. Both factors share the highly conserved DNA binding domain and are bound to DNA sequences with an identical consensus. Here we analyzed the differential gene expression effect mediated by expression of Ctcfl in undifferentiated and differentiated NIH3T3 cell.

2018-05-30 | GSE103196 | GEO

Murine NIH3T3 Cells: Wild vs. BRAFV600E Transfected

Project description:Transcriptional profiling of mouse NIH3T3 cells comparing control NIH3T3 cells transfected with a pEFm6-BRAF with cells transfected with pEFm6-BRAFV600E. Goal was to determine the effects of BRAFV600E gene transfection on global mouse NIH3T3 cells gene expression.

2017-01-01 | GSE90589 | GEO

Apatite nanoparticle(sCA)-siTIMP1 delivery in NIH3T3

Project description:Expression profiling of sCA-siRNA delivery in NIH3T3 cells (siControl vs. siTIMP1) Goal was to determine the off target effects of sCA-siTIMP1 in NIH3T3 cells.

2020-08-27 | GSE128124 | GEO

Transcription profiling of oncovav- and oncovav2-transformed NIH3T3 cells vs parental NIH3T3 controls

Project description:Effect of the overexpression of the oncogenic forms of the Vav2 and Vav proteins in the NIH3T3 cell line. oncovav- and oncovav2-transformed NIH3T3 cells are compared to the parental NIH3T3 controls under exponential growth conditions

2005-06-22 | E-MEXP-135 | biostudies-arrayexpress

Circadian rhythm in NIH3T3 cells

Project description:Identification of cyclical expressed coding and non-coding genes during the circadian rhythm in NIH3T3 cells. NIH3T3 cells were synchronized for their circadian rhythm and RNA sequencing were performed at several time points along the rhythm. This data was used to identify cyclical expressed genes as well as long intergenic non-coding RNAs.

2015-12-31 | GSE66243 | GEO

Phactr1/PP1 phosphatase substrates in mouse NIH3T3 fibroblasts

Project description:It was previously shown that expression of an activated Phactr1 mutant (Phactr1XXX) that constitutively forms the Phactr1/PP1 phosphatase holoenzyme, induces F-actin rearrangements in NIH3T3 fibroblasts. Expression of the Phactr1 PP1-binding domain (C-terminal) alone is also sufficient to induce such cytoskeletal changes. In contrast, expression of Phactr1XXXC derivative, which lacks the PP1 binding sequences does not result in alteration of cytoskeletal morphology (Wiezlak et al., 2012). These observations suggest that Phactr1/PP1 dephosphorylates target proteins involved in cytoskeletal dynamics. To identify potential Phactr1/PP1 substrates, we used differential SILAC phosphoproteomics in NIH3T3 cells inducibly expressing Phactr1XXX, Phactr1XXXC constructs or vector alone. Over 3000 phosphorylation sites were quantified, among which we determined Phactr1/PP1 target dephosphorylation sites by comparative analysis.

2020-10-28 | PXD019977 | Pride

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