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Transcriptome changes triggered by Hyaloperonospora parasitica arabidopsis Noco2 in WT and wrky72 mutants at 96 hpt

ABSTRACT: Functional characterization of AtWRKY72 using Arabidopsis T-DNA insertion lines showed that this gene is important for basal defense to root-knot nematode (RKN) and Hyaloperonospora parasitica arabidopsis (Hpa), but not several tested R gene-mediated defenses.To profile transcriptional reprogramming associated with AtWRKY72-dependent basal defense we used Affymetrix ATH1 GeneChips representing ~24,000 Arabidopsis genes. Three independent biological replicates were performed with Col-0, wrky72-1 and wrky72-2 plants at 96 hpt with HpaNoco2 or mock treatment. Using a false discovery rate of less than 0.05 we identified for each of these three lines genes that showed significant transcriptional changes in response to HpaNoco2 compared to the mock-treated controls. Identification of downstream targets of WRKY72 in Arabidopsis by this microarray suggests that WRKY72 uses a unique signaling pathway that involves AP2/ERF TFs independent of the ethylene signaling pathway.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE18329 | GEO | 2010/08/09

SECONDARY ACCESSION(S): PRJNA117987

REPOSITORIES: GEO

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Transcriptome changes triggered by Hyaloperonospora parasitica arabidopsis Noco2 in WT and wrky72 mutants at 96 hpt

Project description:Functional characterization of AtWRKY72 using Arabidopsis T-DNA insertion lines showed that this gene is important for basal defense to root-knot nematode (RKN) and Hyaloperonospora parasitica arabidopsis (Hpa), but not several tested R gene-mediated defenses.To profile transcriptional reprogramming associated with AtWRKY72-dependent basal defense we used Affymetrix ATH1 GeneChips representing ~24,000 Arabidopsis genes. Three independent biological replicates were performed with Col-0, wrky72-1 and wrky72-2 plants at 96 hpt with HpaNoco2 or mock treatment. Using a false discovery rate of less than 0.05 we identified for each of these three lines genes that showed significant transcriptional changes in response to HpaNoco2 compared to the mock-treated controls. Identification of downstream targets of WRKY72 in Arabidopsis by this microarray suggests that WRKY72 uses a unique signaling pathway that involves AP2/ERF TFs independent of the ethylene signaling pathway. We intended to identify Arabidopsis genes that are regulated in WRKY72-dependent manner in response to Hyaloperonospora parasitica arabidopsis (Hpa) at 96 hpt. For this, we used Col-0 and two wrky72 mutant lines and compared the differentially expressed genes with the hypothesis ‘genes that are specifically regulated in Col-0 but not in wrky72 mutants are WRKY72-dependent genes’.

2010-08-09 | E-GEOD-18329 | biostudies-arrayexpress

Transcription profiling of different Arabidopsis genotypes in a time series after infection with Hyaloperonospora parasitica isolates

Project description:Global transcript profiles of different Arabidopsis thaliana genotypes were examined at 0h, 12h, 24h or 48h post infection with one of three different isolates (races) of the oomycete pathogen Hyaloperonospora parasitica (Hp). The three different isolates of Hp were used for this study, were HpEmoy2, HpHiks1 and HpEmco5 (see Eulgem et al., 2004, Plant Physiol. 135: 1129-1144 for more information). Experimental parameters varied in this study were Arabidopsis genotype, Hp isolate and time after infection.

2007-01-29 | E-TABM-96 | biostudies-arrayexpress

RNA-seq analysis of D-tagatose-treated Arabidopsis thaliana inoculated with Hyaloperonospora arabidopsidis isolate Noco-2

Project description:Rare sugars are monosaccharides that are rarely present in nature. To reveal a effect of D-tagatose in plant and the responses to the D-Tagatose in Arabidopsis inoculated with Hyaloperonospora arabidopsidis (Hpa) isolate Noco-2 at gene expression level, we performed a RNA-seq analysis using the Illumina NGS. As a result, there are no significant differences on the gene expression patterns between in mock- and D-tagatose-treated Arabidopsis plants and Hyaloperonospora arabidopsidis.

2020-08-12 | GSE136568 | GEO

RNA-sequence of Arabidopsis thaliana lines gsnor1 and Col-0 post infection of Phytophthora parasitica against controls

Project description:We performed RNA-Seq mediated transcriptomic analysis of arabidopsis Col-0 and gsnor1-3 seedlings post infection by Phytophthora parasitica/mock to determine the impact of loss of GSNOR1 function on global gene expression following P. parasitica inoculation.

2021-09-30 | E-MTAB-8845 | biostudies-arrayexpress

Transcription profiling of Arabidopsis thaliana Col‑0 wild-type, Col-0 nrpe1-11, and Col-0 ros1-4 at 48 and 72 hours after inoculation with Hyaloperonospora arabidopsidis strain WACO9.

Project description:The experiment was designed to gain more insight into the role of DNA (de)methylation in the regulation of Arabidopsis thaliana gene transcription during a compatible interaction with the downy mildew pathogen Hyaloperonospora arabidopsidis strain WACO9 (Hpa). For this study, comparisons were made between Col-0 (wild-type), nrpe1-11 (which is affected in DNA methylation and globally hypo-methylated) and ros1-4 (impaired in DNA de-methylation and globally hyper-methylated). The latter two mutants have opposite phenotypes with respect to basal resistance and salicylic acid-dependent defence against Hpa. Samples were taken at 48 and 72 hours after spray-inoculation with either 10^5 spores ml^-1 Hyaloperonospora arabidopsidis strain WACO9 in water or water only (mock).

2016-08-03 | E-MTAB-3963 | biostudies-arrayexpress

Arabidopsis thaliana/Hyaloperonospora arabidopsidis compatible interaction transcriptome

Project description:We used Arabidopsis full-genome microarrays to characterize plant transcript accumulations at different stages of infection with the biotrophic oomycete downy mildew pathogen, Hyaloperonospora arabidopsidis : initiation (< 1 dpi) and maintenance of infection (> 4 dpi).

2010-03-26 | GSE21076 | GEO

Arabidopsis thaliana/Phytophthora parasitica compatible interaction transcriptome

Project description:Oomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. Due to particular physiological characteristics, no treatments against diseases caused by oomycetes are presently available. To develop such treatments, it appears essential to dissect the molecular mechanisms that determine the interaction between Phytophthora species and host plants. The present project is focused on the molecular mechanisms that underlie the compatible plant-oomycete interaction and plant disease. The laboratory developed a novel interaction system involving the model plant, Arabidopsis thaliana, and Phytophthora parasitica, a soil-borne pathogen infecting a wide host range, thus representing the majority of Phytophthora species. A characteristic feature of the compatible Arabidopsis/P. parasitica interaction is an extended biotrophic phase, before infection becomes necrotrophic. Because the initial biotrophic phase is extremely short on natural (e.g. solanaceous) hosts, the Arabidopsis system provides the opportunity to analyze, for both interaction partners, the molecular events that determine the initiation of infection and the switch to necrotrophy. The present project aims at analyzing the compatible interaction between A. thaliana roots and P. parasitica. The Affymetrix A. thaliana full genome chip will be used to characterize modulations of the transcriptome occurring over a period of 24h from the onset of plant root infection to the beginning of necrotrophy. Parallel to this study, a custom-designed P. parasitica biochip will enable analyzing of P. parasitica gene expression during the same stages.

2010-04-20 | GSE20226 | GEO

Transcriptome profile of Carrizo citrange roots in response to Phytophthora parasitica infection

Project description:Phytophthora parasitica is one of the most widespread Phytophthora species, which is known to cause root rot, foot rot/gummosis and brown rot of fruits in citrus. In this study, we have analyzed the transcriptome of a commonly used citrus rootstock Carrizo citrange in response to P. parasitica infection using the RNA-seq technology. In total, we have identified 6692 differentially expressed transcripts (DETs) among P. parasitica-inoculated and mock-treated roots. Of these, 3960 genes were differentially expressed at 24 hours post inoculation and 5521 genes were differentially expressed at 48 hours post inoculation. Gene ontology analysis of DETs suggested substantial transcriptional reprogramming of diverse cellular processes particularly the biotic stress response pathways in Carrizo citrange roots. Many R genes, transcription factors, and several other genes putatively involved in plant immunity were differentially modulated in citrus roots in response to P. parasitica infection. Analysis reported here lays out a strong foundation for future studies aimed at improving resistance of citrus rootstocks to P. parasitica.

2019-04-13 | GSE129758 | GEO

Expression data from 2-week-old Arabidopsis untreated seedlings grown under a short day condition

Project description:The Arabidopsis thaliana defense regulator EDM2 was previously shown to be specifically required for disease resistance to the pathogenic oomycete Hyaloperonospora parasitica aradidopsis mediated by the R protein RPP7. We found EDM2 to have a promoting effect on several distinct developmental processes, such as leaf pavement cell development, vegetative phase change or the floral transition. We further identified the atypical protein kinase WNK8 to physically interact with EDM2 in nuclei. Microarray data support regulatory interactions between WNK8 and EDM2 as well as their functional association with the autonomous floral promotion pathway.

2010-09-21 | GSE17499 | GEO

Arabidopsis thaliana/Phytophthora parasitica compatible interaction transcriptome

2010-04-28 | E-GEOD-20226 | biostudies-arrayexpress

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