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Anaerobic carbon-limited retentostat cultivation of Lactobacillus plantarum WCFS1

ABSTRACT: Lactobacillus plantarum WCFS1 was grown under anaerobic carbon-limited conditions in a chemostat with complete biomass retention (retentostat). In this cultivation system, the biomass concentration progressively increases while the dilution rate is kept constant, resulting in decreased specific susbtrate availibility, and hence, a progressive decrease in the specific growth rate. During the progressive transition from growth to virtually no growth, the global changes occurring at the level of metabolism and gene expression were studied using a genome-scale metabolic model and DNA microarrays.

ORGANISM(S): Lactiplantibacillus plantarum WCFS1 Lactiplantibacillus plantarum

PROVIDER: GSE18340 | GEO | 2010/01/01

SECONDARY ACCESSION(S): PRJNA118009

REPOSITORIES: GEO

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Anaerobic carbon-limited retentostat cultivation of Lactobacillus plantarum WCFS1

Project description:Lactobacillus plantarum WCFS1 was grown under anaerobic carbon-limited conditions in a chemostat with complete biomass retention (retentostat). In this cultivation system, the biomass concentration progressively increases while the dilution rate is kept constant, resulting in decreased specific susbtrate availibility, and hence, a progressive decrease in the specific growth rate. During the progressive transition from growth to virtually no growth, the global changes occurring at the level of metabolism and gene expression were studied using a genome-scale metabolic model and DNA microarrays. Four different time-points are compared, corresponding to 4 different specific growth rates, and hence, 4 different ratios of energy used for maintenance and growth. The samples taken at the start of retentostat cultivation serves as a a reference sample, to which the three other samples (taken after 3 days, 17 days, and 31 days under retentostat conditions) are compared. No biological replicates: all samples were taken from the same retentostat fermentation.

2010-05-16 | E-GEOD-18340 | biostudies-arrayexpress

Transcriptional profile Lactobacillus plantarum WCFS1 : Oleuropein treated cells

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Ethanol stress response in Lactobacillus plantarum WCFS1

Project description:Transcriptome profiles of control Lactobacillus plantarum WCFS1 cells were compared with 8% ethanol adapted cells and with 10 min or 30 min 8% ethanol shocked cells.

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Sugar utilization in L. plantarum WCFS1

Project description:Lactobacillus plantarum was grown anaerobically on 4 different sugars (Mannose Lactose Fructose and Sucrose) to OD600 = 1.0. Samples were compared with a similar grown culture on glucose. An independnet biological duplicate of tht experimnet was performed (samples 1 and 2). L. plantarum WCFS1 grown in glucose, mannose, fructose, and sucrose

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Locust bean gum derived galacto-manno-oligosaccharides utilization by Lactobacillus plantarum WCFS1

Project description:In order to understand LBG derived galacto-manno-oligosaccharides utilization by a probiotic bacterium, Lactobacillus plantarum WCFS1, we have grown Lactobacillus plantarum WCFS1 (in duplicates) till mid log phase (OD600nm ~0.5, 10 h) in carbon free MRS (de Man, Rogosa Sharpe ) media containing either galacto-manno-oligosaccharides, mannose, glucose or galactose (1% w/v) as the sole carbon source.

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Transcriptional profile Lactobacillus plantarum WCFS1 : p-coumaric acid (p-CA) treated cells

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Project description:Transcriptome profiles of control Lactobacillus plantarum WCFS1 cells were compared with 8% ethanol adapted cells and with 10 min or 30 min 8% ethanol shocked cells. A continuous culture was performed in duplicate on medium containing ehtanol (tE). A shock experiment was performed in duplicate in which ethanol was added to a batch culture immediately after t0 (=control). The culture was sampled at time=0 (t0), time =10 min (t1) and time=30 min (t2).

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Transcriptional profile Lactobacillus plantarum WCFS1 : olive oil (OO) treated cells

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Transcriptional profile Lactobacillus plantarum WCFS1 : Hydroxytyrosol treated cells

Project description:Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with hydroxytyrosol. Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with hydroxytyrosol. Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. The transcriptional profile reveals the induction of genes related to defense mechanisms against oxidative challenge. This response include genes related to inactivation of toxic oxygen radicals, probably derived from the autooxidation of hydroxytyrosol and genes involved in repairing the damage of oxygen radicals on proteins, including those involved in the metabolism of sulphur amino acids. Genes involved in peptidoglycan turnover, strenghthening of the cell wall and genes coding for osmoprotectants transporters, were downregulated. In addition, genes coding for elements typically involved in the stringent response, including ribosomal proteins, enzymes involved in the metabolism of the ppGpp alarmone, nitrogen metabolism, cell division and general stress proteins, were differentially regulated by hydroxytyrosol.

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Lactiplantibacillus plantarum WCFS1

Project description:Ethanol stress response in Lactobacillus plantarum WCFS1

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