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Maternal Smchd1 regulates Hox gene expression and patterning in the mouse embryo [RNAseq tailbud]

ABSTRACT: RNAseq from somite-matched tailbud tissue from E8-8.5 embryo tailbuds at the 6-11 somite developmental stages

ORGANISM(S): Mus musculus

PROVIDER: GSE183733 | GEO | 2022/06/27

REPOSITORIES: GEO

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Maternal SMCHD1 regulates Hox gene expression and patterning in the mouse embryo.

Project description: Not available

| S-EPMC9314430 | biostudies-literature

Maternal Smchd1 regulates Hox gene expression and patterning in the mouse embryo [RNAseq tailbud]

Project description:Maternal Smchd1 regulates Hox gene expression and patterning in the mouse embryo [RNAseq tailbud]

| PRJNA761774 | ENA

Capture-C in somite-matched pre-somitic mesoderm from Smchd1GFP/GFP, Smchd1MD43-GFP/MD43-GFP, Smchd1+/+ and Smchd1MD43/MD43 embryos.

Project description:Capture-C from in situ HiC libraries in tailbud tissue containing pre-somitic mesoderm (PSM) dissected from Smchd1GFP/GFP, Smchd1MD43-GFP/MD43-GFP, Smchd1+/+ and Smchd1MD43/MD43 embryos E8.5 embryos at the 7-9 somite stage of development. Somite stage is a good indicator of the precise stage of embryonic development.

2023-04-04 | GSE228673 | GEO

RNAseq from 8 somite stage PSM

Project description:RNAseq from somite-paired pre-somitic mesoderm from E8.5 embryo tailbuds at the 8-somite developmental stage

2021-05-08 | GSE174069 | GEO

Maternal Smchd1 regulates Hox gene expression and patterning in the mouse embryo

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-06-27 | GSE183740 | GEO

scRNA-seq of tailbud development in zebrafish

Project description:We have performed single-cell RNA-seq of whole tailbuds in WT zebrafish at 16 hours post fertilization (hpf).

2023-12-31 | GSE171482 | GEO

Maternal Smchd1 regulates Hox gene expression and patterning in the mouse embryo [RNAseq mESC]

Project description:Parents transmit genetic and epigenetic information to their offspring. Maternal effect genes regulate the offspring epigenome to ensure normal development. Here we report that the epigenetic regulator SMCHD1 has a maternal effect on Hox gene expression and skeletal patterning. Maternal SMCHD1, present in the oocyte and preimplantation embryo, prevents precocious activation of Hox genes post-implantation. Without maternal SMCHD1, highly penetrant posterior homeotic transformations occur in the embryo. Hox genes are decorated with Polycomb marks H2AK119ub and H3K27me3 from the oocyte throughout early embryonic development; however, loss of maternal SMCHD1 does not deplete these marks. Therefore, we propose maternal SMCHD1 acts downstream of Polycomb marks to establish a chromatin state necessary for persistent epigenetic silencing and appropriate Hox gene expression later in the developing embryo. This is a striking role for maternal SMCHD1 in long-lived epigenetic effects impacting offspring phenotype.

2022-06-27 | GSE183736 | GEO

Maternal Smchd1 regulates Hox gene expression and patterning in the mouse embryo [ESC RRBS]

2022-06-27 | GSE203617 | GEO

Single-cell RNA-seq of tailbuds from control, Pbx1/Pbx2 compound and Pbx1/Pbx2 double-knockout E8.5/E9.0 embryos.

Project description:In vertebrates, body axis elongation is fuelled by bipotent neuromesodermal progenitors (NMPs), which support the development of both spinal cord and paraxial mesoderm (PM). NMPs reside in the caudal lateral epiblast and tailbud, from where they sustain axial elongation. HOX transcription factors have been historically implicated in axial elongation, with their sequential activation playing a fundamental role in timing PM development. PBX1 and PBX2 are obligate anterior HOX cofactors, and therefore they represent prominent candidates for controlling the distinct response to individual HOX factors. To pinpoint the role of PBX proteins in the development of pre-somitic mesoderm, single-cell RNA sequencing (scRNA-seq) was performed on cells isolated from the tailbuds of control, Pbx1/Pbx2 compound (Pbx1/2-com) and Pbx1/Pbx2 double-mutant (Pbx1/2-DKO) embryos at embryonic days 8.5 and 9.0. Single cells from at least three embryos per genotype and stage were FACS-sorted into 384-well capture plates, and scRNA-seq was performed using MARS-seq (Jaitin D.A. et al., Science, 343, 776-779 (2014)). As a spike-in internal control for batch-effect correction, 71 EpiSCs cultivated in vitro were sorted into each plate, together with 311 cells from embryonic tailbuds. Two wells were left empty in each plate, as a no-cell control during data analysis.

2021-07-21 | E-MTAB-9785 | biostudies-arrayexpress

Maternal Smchd1 regulates Hox gene expression and patterning in the mouse embryo [H2AK119ub CUT&RUN]

2022-06-27 | GSE183739 | GEO

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