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Comparison of bovine undifferentiated MSCs, chondrogenic MSCs and chondrocytes in 3D culture

ABSTRACT: Bovine chondrocyte-seeded and mesenchymal stem cell (MSC)-seeded agarose were cultured for 28 days in chemically defined media containing 10 ng/mL TGF-beta3. Chondrogenic differentiated MSCs were compared to chondrocytes at this timepoint and to undifferentiated MSCs harvested at day 0.

ORGANISM(S): Bos taurus

PROVIDER: GSE18394 | GEO | 2010/10/02

SECONDARY ACCESSION(S): PRJNA118081

REPOSITORIES: GEO

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Comparison of bovine undifferentiated MSCs, chondrogenic MSCs and chondrocytes in 3D culture

Project description:Bovine chondrocyte-seeded and mesenchymal stem cell (MSC)-seeded agarose were cultured for 28 days in chemically defined media containing 10 ng/mL TGF-beta3. Chondrogenic differentiated MSCs were compared to chondrocytes at this timepoint and to undifferentiated MSCs harvested at day 0. Donor-matched sets of chondrocytes and MSCs were used, with three donors total.

2010-10-02 | E-GEOD-18394 | biostudies-arrayexpress

Effects of dynamic compressive loading on MSC chondrogenesis

Project description:Long-term dynamic compression enhanced the mechanical properties of MSC-seeded constructs only when loading was initiated after 21 days of chondrogenic differentiation. This study examined the molecular differences of chondrogenic MSCs compared to undifferentiated MSCs (TGF-beta vs no TGF-beta) and the effects of dynamic loading on MSC chondrogenesis (loading vs free-swelling). Free-swelling MSC-seeded constructs were cultured for 21 days in chemically defined media. Chondrogenesis was induced with TGF-beta3. Undifferentiated controls were maintained in parallel. After 21 days of chondrogenic differentiation, a subset of constructs were subjected to 21 days of dynamic compressive loading. On days 21 and 42, construct mechanical properties and biochemical content were assessed. Microarray analysis was carried out on day 3, day 21 and day 42 constructs. 6 arrays.

2010-11-01 | E-GEOD-18879 | biostudies-arrayexpress

Gene array comparison of highly and poorly chondrogenic clones of adult human mesenchymal stem cells (MSCs)

Project description:MSCs are a heterogeneous population of cells with different capacities for lineage differentiation. MSC clones were prepared from a single adult human bone marrow sample and screened for their chondrogenic capacity using cartilage tissue engineering (measured by type II collagen content). The aim was to compare genes expressed by highly and poorly chondrogenic clones to identify genes associated with those MSCs with an enhanced capacity for cartilage formation. The 4 most highly chondrogenic and the 4 least chondrogenic MSC clones identified following screening by cartilage tissue engineering were selected for gene array comparison using Affymetrix microarrays.

2015-04-30 | E-GEOD-54684 | biostudies-arrayexpress

Differential protein expression between chondrogenic differentiated MSCs, undifferentiated MSCs and adult chondrocytes derived from Oryctolagus cuniculus in vitro.

Project description: Not available

| S-EPMC3880988 | biostudies-literature

Gene array comparison of highly and poorly chondrogenic clones of adult human mesenchymal stem cells (MSCs)

2015-04-30 | GSE54684 | GEO

Expression difference between osteoarthritic chondrocytes and mesenchymal stem cells during chondrogenic differentiation

Project description:The recruitment of mesenchymal stem cells in order to reconstruct damaged cartilage of osteoarthritis joints is a challenging tissue engineering task. Vision towards this goal is blurred by a lack of knowledge about the underlying differences between chondrocytes and MSC during the chondrogenic cultivation process. The aim of this study was to shed light on the differences between chondrocytes and MSC occurring during chondral differentiation through tissue engineering. As a model we used the pellet culture system under chondrogenic conditions for the comparison of chondrocyte and MSC differentiation. Immunohistology was followed by microarray analysis, which was filtered through already published datasets describing different developmental processes. Validation was performed with quantitative RT-PCR. Results describe inferior chondrogenic ECM-production by MSCs and underline their closer link to the osteogenic lineage. Chondrocytes have an upregulated fatty acid/cholesterol metabolism which might give hints for future modifications of culture conditions. To shed light on the differences between chondrocytes and MSC occurring during chondral differentiation through tissue engineering, a pellet culture system under chondrogenic conditions for the comparison of chondrocyte and MSC differentiation was used after 0, 3, 7 and 14 days

2010-05-14 | E-GEOD-19664 | biostudies-arrayexpress

Expression difference between osteoarthritic chondrocytes and mesenchymal stem cells during chondrogenic differentiation

2010-03-01 | GSE19664 | GEO

mRNAs and lncRNAs expression data of chondrogenic differentiated and undifferentiated murine chondrogenic cell line ATDC5 cells

Project description:Chondrogenic differentiation is a coordinated biological process regulated by various cell signalings at both transcriptional and post-transcriptional levels. In this study, microarray was performed to detect the expression profiles of lncRNAs and mRNAs during chondrogenic differentiation of murine chondrogenic cell line ATDC5.

2018-12-28 | GSE124424 | GEO

Effects of dynamic compressive loading on MSC chondrogenesis

2010-11-01 | GSE18879 | GEO

Differential expression of human BM-MSCs RNA-Seq and 21-day of chondrogenic differentiation from high-depth transcriptomic profiling of mesenchymal stem cells during chondrogenesis

Project description:A comprehensive evaluation of differential gene expression on RNA-seq data of early and late passage hMSCs from three individuals, and undifferentiated and 21-day of chondrogenic differentiation hMSCs from previous publication (GSE109503). Genome-wide RNA-seq and differential expression analysis revealed that signalings such as Pulmonary Fibrosis Idiopathic (IPF) Signaling Pathway and Osteoclasts in Rheumatoid Arthritis (RA) Signaling Pathway were significant differences in early MSC. Differential FOXO1 expression, higher in Early than Late MSCs, was responsible for promoting suspension survival, and cell migration, and induces chondrogenic differentiation.

2023-02-24 | GSE225530 | GEO

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