The DNMT1 inhibitor GSK-3484862 mediates global demethylation in murine embryonic stem cells

ABSTRACT: We generated WGBS datasets from mESCs treated with the novel drug GSK-3484862 attained from two different commercial sources or the conventional DNMT1 inhitibitor 5-azacytidine. To assess efficacy, datasets were generated from mESCs treated with GSK-3484862 at two concentrations and after treatment for 6 days and 14 days each. Additionally, for comparison purposes, we generated WGBS datasets from Dnmt1/3a/3b deficient mESCs which have very low levels of DNA methylation and WT mESCs treated with DMSO which retain high levels of DNA methylation.

ORGANISM(S): Mus musculus

PROVIDER: GSE184116 | GEO | 2021/12/14

REPOSITORIES: GEO