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Drosophila photoreceptor transcriptome landscape upon disruption of Clock activity [Rh1-ClkDN_RNA]

ABSTRACT: We report the transcriptome landscape of Drosophila photoreceptor neurons obtained using a tissue-specific approach at different time points after eclosion upon expression of a Clock dominant negative mutant

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE184121 | GEO | 2021/12/31

REPOSITORIES: GEO

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Drosophila photoreceptor chromatin accessibility landscape upon disruption of Clock activity [ClkATACseq]

Project description:We report the chromatin accessibility landscape of Drosophila photoreceptor neurons obtained using a tissue-specific approach at different time points after eclosion upon expression of a Clock dominant negative mutant

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Drosophila photoreceptor chromatin accessibility landscape upon disruption of Clock activity [ClkATACseq]

Project description:Drosophila photoreceptor chromatin accessibility landscape upon disruption of Clock activity [ClkATACseq]

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Drosophila photoreceptor transcriptome landscape upon disruption of Clock activity [Rh1-ClkDN_RNA]

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Core Circadian Clock Genes Per1 and Per2 regulate the Rhythm in Photoreceptor Outer Segment Phagocytosis [Photoreceptor]

Project description:Core Circadian Clock Genes Per1 and Per2 regulate the Rhythm in Photoreceptor Outer Segment Phagocytosis [Photoreceptor]

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Core Circadian Clock Genes Per1 and Per2 regulate the Rhythm in Photoreceptor Outer Segment Phagocytosis [Photoreceptor]

Project description:Retinal photoreceptors undergo daily renewal of their distal outer segments, a process indispensable for maintaining retinal health. Photoreceptor Outer Segment (POS) phagocytosis occurs as a daily peak, roughly about one hour after light onset. However, the underlying cellular and molecular mechanisms which initiate this process are still unknown. Here we show that, under constant darkness, mice deficient for core circadian clock genes (Per1 and Per2), lack a daily peak in POS phagocytosis. By qPCR analysis we found that core clock genes were rhythmic over 24h in both WT and Per1, Per2 double mutant whole retinas. More precise transcriptomics analysis of laser capture microdissected WT photoreceptors revealed no differentially expressed genes between time-points preceding and during the peak of POS phagocytosis. By contrast, we found that microdissected WT retinal pigment epithelium (RPE) had a number of genes that were differentially expressed at the peak phagocytic time-point compared to adjacent ones. We also found a number of differentially expressed genes in Per1, Per2 double mutant RPE compared to WT ones at the peak phagocytic time-point. Finally, based on STRING analysis we found a group of interacting genes which potentially drive POS phagocytosis in the RPE. This potential pathway consists of genes such as: Pacsin1, Syp, Camk2b and Camk2d among others. Our findings indicate that Per1 and Per2 are necessary clock components for driving POS phagocytosis and suggest that this process is transcriptionally driven by the RPE.

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Transcriptional profiling of aged Drosophila photoreceptors

Project description:Purpose: The goal of this study was to globally characterize the transcript levels of genes in Drosophila photoreceptor neurons during aging. Using transcriptome profiling of isolated photoreceptor nuclei we analyzed the changes in gene expression that occur during aging over 5 time-points from 10 to 40 days post-eclosion and identified sets of genes with both up-regulated and down-regulated expression.

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GIGANTEA recruits deubiquitylases, UBP12 and UBP13, to regulate accumulation of the ZTL photoreceptor complex

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