Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Excess S-Adenosylmethionine inhibits methylation via catabolism to adenine

ABSTRACT: Mouse embryonic fibroblasts were treated with 1mM SAM, 1mM adenine or 10microM, and transcriptome signatures were compared to untreated cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE184525 | GEO | 2021/09/23

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Excess S-Adenosylmethionine inhibits methylation via catabolism to adenine

Project description:Excess S-Adenosylmethionine inhibits methylation via catabolism to adenine

| PRJNA764914 | ENA

S-adenosylmethionine (SAM) changes DNA methylation patterns in prostate cancer cells

Project description:Genome-wide DNA methylation profiling of human PC3 invasive prostate cancer cell line treated with vehicle control (SAH, S-adenosylhomocysteine) and with SAM (S-adenosylmethionine) as well as of untreated human LNCaP non-invasive prostate cancer cell line. The Illumina Infinium 450k Human DNA Methylation BeadChip v1.2 was used to obtain DNA methylation profiles across approximately 450,000 CpGs in human cell lines exposed to described treatments. Samples included biological triplicate of PC3 control (SAH treated), biological triplicate of PC3 treated with SAM, and biological duplicate of LNCaP untreated.

2015-03-01 | GSE62053 | GEO

S-adenosylmethionine (SAM) changes DNA methylation patterns in prostate cancer cells

2015-03-01 | E-GEOD-62053 | biostudies-arrayexpress

Nanopore RNA-seq of human cell lines treated with or without nicotinamide adenine dinucleotide (NAD+)

Project description:Investigation of transcriptome changes in four human cell lines (BJ, BJ-5ta, U2OS and HeLa) after treatment for 24 hours with nicotinamide adenine dinucleotide (NAD+). Cells were untreated as the control condition. Nanopore sequencing of cDNA was performed after library preparation with the ONT SQK-PCB109 kit.

2023-09-20 | E-MTAB-13187 | biostudies-arrayexpress

Adenine effect on Enterohemorraghic E.coli

Project description:Previous experiments have shown that E. feacalis increases EHEC virulence by secreting adenine, this RNAseq aims to understand the molecular mechanism underlaying adenine role on EHEC

2024-05-01 | GSE244178 | GEO

Formaldehyde regulates S-adenosylmethionine biosynthesis and one-carbon metabolism

Project description:One-carbon metabolism is an essential branch of cellular metabolism that intersects with epigenetic regulation. Here, we show formaldehyde, a one-carbon unit derived from both endogenous sources and environmental exposure, regulates one-carbon metabolism by inhibiting the biosynthesis of S-adenosylmethionine (SAM), the major methyl donor in cells. Formaldehyde reacts with privileged, hyperreactive cysteine sites in the proteome, including Cys120 in S-adenosylmethionine synthase isoform type-1 (MAT1A). Formaldehyde exposure inhibited MAT1A activity and decreased SAM production with MAT-isoform specificity. A genetic mouse model of chronic formaldehyde overload showed a decrease in SAM and in methylation on selected histones and genes. Epigenetic and transcriptional regulation of Mat1a and related genes function as compensatory mechanisms for formaldehyde-dependent SAM depletion, revealing a biochemical feedback cycle between formaldehyde and SAM one-carbon units.

2023-09-05 | GSE242266 | GEO

Transcriptome analysis of adenine-treated murine microglial cells

Project description:In previous study, we demonstrated adenine-induced cell signaling on anti-inflammation in BV2 cells. To investigate the changes of transcriptome mediated by adenine, we used microarrays to compare the difference in expression levels.

2017-08-01 | GSE84217 | GEO

Formaldehyde regulates one-carbon metabolism through S-adenosylmethionine biosynthesis

Project description:One-carbon metabolism is a universal hub for cellular metabolism and epigenetic regulation.1–3 Here, we report that formaldehyde (FA), a one-carbon unit that organisms produce in substantial quantities through folate metabolism,4 is a regulator of the one-carbon cycle via the biosynthesis of S-adenosyl-L-methionine (SAM), an essential one-carbon building block for synthesis of nucleotides, amino acids, and methylated nucleic acids and proteins.5 Activity-based protein profiling (ABPP) in mouse liver tissue identifies FA-sensitive cysteine sites across the proteome, revealing several one-carbon cycle targets including S-adenosylmethionine synthetase isoform 1 (MAT1A), the terminal enzyme in SAM biosynthesis. Biochemical studies of the formaldehyde-MAT1A interaction establish FA-dependent inhibition of MAT1A activity through a conserved C120 site, as the MAT2A isoform lacking this cysteine is not FA-sensitive. CRISPR knockout-generated HepG2 cell models that predominantly express either MAT1A or MAT2A show that MAT1A-positive cells respond to FA treatment in a dose-dependent manner by decreasing their SAM levels and downstream RNA methylation, whereas the MAT2A-positive cells are not affected by FA. Our findings reveal an unexpected interplay between SAM and FA, two central one-carbon units to influence the overall methylation potential of the cell.

2023-11-02 | PXD018251 | Pride

Transcription profiling of Arabidopsis seedlings treated with 6-Benzyl adenine - time series

Project description:Time course of Arabidopsis seedlings treated with 6-Benzyl adenine dissolved in diluted KOH for 15 min or 2 hours

2008-06-11 | E-GEOD-1766 | biostudies-arrayexpress

S-adenosylmethionine (SAM) treatment attenuates breast cancer growth, invasion, metastasis

Project description:This study examines the therapeutic plausibility of using universal methyl group donor S-adenosylmethionine (SAM) to block breast cancer development, growth, and metastasis. cancer. Anti-tumor and anti-metastatic activity of SAM was evaluated through a series of studies in vitro using two different human breast cancer cell lines and in vivo using a MDA-MB-231 xenograft model of breast cancer. The data shown in this array is obtained from control and SAM-treated MDA-MB-231 cell lines.

2017-04-28 | GSE98275 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data