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Argonaute immunoprecipitation reveals mis-annotation of human microRNAs

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE184535 | GEO | 2025/04/23

REPOSITORIES: GEO

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Argonaute immunoprecipitation reveals mis-annotation of human microRNAs [miRNA-seq]

Project description:MicroRNAs (miRNAs) form the sequence recognition component of the RNA-induced silencing complex (RISC), bringing Argonaute (AGO) and other RISC-associated proteins to target transcripts where they mediate post-transcriptional gene silencing. High-throughput sequencing has helped to catalogue several thousand human miRNAs, as well as a plethora of other small RNAs derived from larger transcripts. Here, we take a closer inspection of sequencing data derived from cellular fractionation and AGO-RNA immunoprecipitation and find that a subset of small RNAs, despite being denoted as miRNAs, are in fact almost exclusively nuclear and do not associate with AGO. Experimental validation of a select number of these small RNAs also fails to show endogenous miRNA-like activity, suggesting they are degradation products from larger transcripts that have been mis-annotated. Despite this, the presumed miRNA functions of almost 20 of these have collectively been the subject of several hundred publications, emphasising the need to filter unlikely miRNA candidates from sequencing data and large miRNA-sequence repositories.

2025-04-23 | GSE184534 | GEO

Argonaute immunoprecipitation reveals mis-annotation of human microRNAs [HITS-CLIP]

2025-04-23 | GSE184533 | GEO

Argonaute immunoprecipitation reveals mis-annotation of human microRNAs

Project description:Argonaute immunoprecipitation reveals mis-annotation of human microRNAs

| PRJNA764930 | ENA

Argonaute immunoprecipitation reveals mis-annotation of human microRNAs [miRNA-seq]

Project description:Argonaute immunoprecipitation reveals mis-annotation of human microRNAs [miRNA-seq]

| PRJNA764934 | ENA

Argonaute immunoprecipitation reveals mis-annotation of human microRNAs [HITS-CLIP]

Project description:Argonaute immunoprecipitation reveals mis-annotation of human microRNAs [HITS-CLIP]

| PRJNA764932 | ENA

Argonaute 2

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-05-31 | E-GEOD-41081 | biostudies-arrayexpress

Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-05-01 | E-GEOD-45812 | biostudies-arrayexpress

Arabidopsis lyrata gene annotation using RNA-sequencing data

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Cell fractionation reveals mis-annotation of human microRNAs

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Combined microRNA/mRNA transcriptome analysis reveals angiogenic microRNAs-genes pathway of human late endothelial precursor cells.

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